Comparison of the transcriptomic analysis between two Chinese white pear (Pyrus bretschneideri Rehd.) genotypes of different stone cells contents

PloS One
Jinyun ZhangYongping Cai

Abstract

Stone cell content is thought to be one of the key determinants for fruit quality in pears. However, the molecular mechanism of stone cell development remains poorly understood. In this study, we found that the stone cell clusters (SCCs) distribution and area in 'Dangshan Su' (with abundant stone cells) were higher as compared to 'Lianglizaosu' (low stone cell content bud sport of 'Dangshan Su') based on the histochemical staining, and the correlations of lignin content with stone cell content and SCC area was significant. The fruits of 'Dangshan Su' and 'Lianglizaosu' at three different developmental stages (23 and 55 days after flowering and mature) were sampled for comparative transcriptome analysis to explore the metabolic pathways associated with stone cell development. A total of 42444 unigenes were obtained from two varieties, among which 7203 differentially expressed genes (DEGs) were identified by comparison of the six transcriptomes. Specifically, many DEGs associated with lignin biosynthesis were identified, including coumaroylquinate 3-monooxygenase (C3H), shikimate O-hydroxycinnamoyltransferase (HCT), ferulate 5-hydroxylase (F5H), cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POD), as well as genes related t...Continue Reading

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Citations

Feb 26, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Guohui LiYongping Cai

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Datasets Mentioned

BETA
SRR5965144
SRR5965145
AF234300.1
EHA105

Methods Mentioned

BETA
electrophoresis
Illumina sequencing
RNA-seq
PCR
glycosylation

Software Mentioned

BLAST
Excel
BLASTX
SPSS
IDEG6
Trinity assemble
Statistical Program for Social Sciences

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