Comparison of three commercially available dengue NS1 antigen capture assays for acute diagnosis of dengue in Brazil.

PLoS Neglected Tropical Diseases
Monique da Rocha Queiroz LimaFlavia Barreto dos Santos

Abstract

Dengue is associated with explosive urban epidemics and has become a major public health problem in many tropical developing countries, including Brazil. The laboratory diagnosis of dengue can be carried out using several approaches, however sensitive and specific assays useful to diagnose in the early stage of fever are desirable. The flavivirus non-structural protein NS1, a highly conserved and secreted glycoprotein, is a candidate protein for rapid diagnosis of dengue in endemic countries. We aimed to evaluate the potential use of 3 commercial kits in a panel of 450 serum samples for early diagnosis of dengue in Brazil. The PanBio Early ELISA (PanBio Diagnostics) showed a sensitivity of 72.3% (159/220) and a specificity of 100%, while the sensitivity of the Platelia NS1 assay (Biorad Laboratories) was 83.6% (184/220). However, the highest sensitivity (89.6%; 197/220) was obtained by using the NS1 Ag Strip (Biorad Laboratories). A lower sensitivity was observed in DENV-3 cases by all 3 kits. Serum positive by virus isolation were more often positive than cases positive by RT-PCR by all three assays and a higher detection rate was observed during the first four days after the onset of the symptoms. The presence or absence of I...Continue Reading

References

Jan 1, 1990·Annual Review of Microbiology·T J ChambersC M Rice
Jan 1, 1984·The American Journal of Tropical Medicine and Hygiene·D J GublerA Oliver
Dec 30, 1999·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·M P MiagostovichV Vorndam
Sep 6, 2003·Journal of Clinical Microbiology·Penelopie KorakaAlbert D M E Osterhaus
Oct 7, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Stefan SchillingHerbert Schmitz
Sep 22, 2006·Clinical and Vaccine Immunology : CVI·Philippe DussartLaurence Baril
Aug 24, 2007·Clinical and Vaccine Immunology : CVI·Stuart D BlacksellPaul N Newton
Dec 29, 2007·The Pediatric Infectious Disease Journal·Ampaiwan ChuansumritSutee Yoksan
Jan 15, 2008·Diagnostic Microbiology and Infectious Disease·Keswadee LapphraVisanu Thamlikitkul
Apr 24, 2008·Annual Review of Microbiology·Jennifer L Kyle, Eva Harris
Feb 24, 2009·Diagnostic Microbiology and Infectious Disease·Wathanee ChaiyaratanaSutee Yoksan
Feb 24, 2009·Diagnostic Microbiology and Infectious Disease·William John Hannan McBride
Feb 24, 2009·Diagnostic Microbiology and Infectious Disease·Hoang Lan PhuongPeter J de Vries
Apr 28, 2009·Diagnostic Microbiology and Infectious Disease·Subhash C Arya, Nirmala Agarwal
Sep 8, 2009·Diagnostic Microbiology and Infectious Disease·Alvaro H RamirezFerdinando Liprandi

❮ Previous
Next ❯

Citations

Mar 4, 2014·TheScientificWorldJournal·Siraj A KhanJagadish Mahanta
Apr 26, 2013·Applied Microbiology and Biotechnology·Franciele Roberta MaldanerTatsuya Nagata
Sep 6, 2014·Journal of Global Infectious Diseases·Baijayantimala MishraRadha K Ratho
Sep 23, 2014·Transactions of the Royal Society of Tropical Medicine and Hygiene·Thamarasi N Senaratne, Faseeha Noordeen
Sep 17, 2014·The Journal of Infection·Lilly M Verhagen, Ronald de Groot
May 17, 2011·PLoS Neglected Tropical Diseases·Monique da Rocha Queiroz LimaFlavia Barreto dos Santos
Jan 11, 2012·The American Journal of Tropical Medicine and Hygiene·Séverine MatheusPhilippe Dussart
Jan 29, 2013·Neurology International·Marzia Puccioni-SohlerCristiane N Soares
May 30, 2013·PloS One·Vanessa Ramos Faria SeaRoseli La Corte dos Santos
Aug 24, 2013·BMC Infectious Diseases·Boon-Teong TeohSazaly AbuBakar
Jul 6, 2013·Biomedical Microdevices·Naga Siva Kumar GundaSushanta K Mitra
Mar 28, 2013·Expert Opinion on Medical Diagnostics·Anusyah Rathakrishnan, Shamala Devi Sekaran
Oct 23, 2015·Viruses·Om Parkash, Rafidah Hanim Shueb
Oct 9, 2012·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Chung-Hao HuangHo-Sheng Wu
Jan 30, 2016·BMC Infectious Diseases·Sibelle Nogueira BuonoraRegina Paiva Daumas
Mar 15, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Je-Hyoung KimHyun Park
May 12, 2012·Journal of Virological Methods·David A MullerPaul R Young
Oct 30, 2013·Journal of Travel Medicine·Irani RatnamJoseph Torresi
Jun 30, 2015·Journal of Medical Virology·Xinliang ChenHaiyan Yan
Sep 26, 2015·Infectious Diseases of Poverty·N D B EhelepolaMalani Wickramasinghe
Sep 29, 2015·BioMed Research International·Rafaelle C G FaresMaria Rios
Apr 14, 2016·Journal of Medical Entomology·P P BarbosaC F J Ayres
Nov 22, 2011·Journal of Immunological Methods·Elisângela M LinaresStefan Thalhammer
Mar 25, 2014·Diagnostic Microbiology and Infectious Disease·Neil W AndersonElitza S Theel
Jan 31, 2015·Transactions of the Royal Society of Tropical Medicine and Hygiene·Manoela HeringerFlávia B dos Santos
Jan 5, 2014·PLoS Neglected Tropical Diseases·Maria Glória TeixeiraGraham Joint
May 2, 2017·Emerging Infectious Diseases·Anastácio Q SousaMargarida M L Pompeu
Nov 14, 2012·Memórias do Instituto Oswaldo Cruz·Márcia Gonçalves de CastroFlávia Barreto dos Santos
Nov 28, 2016·Jornal de pediatria·Flávio da Silva MesquitaEdison Luiz Durigon
Jul 2, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Hao ZhangYuanping Zhou
May 16, 2012·British Journal of Hospital Medicine·Catherine H RobertsGavin Screaton
Apr 29, 2011·Clinical and Vaccine Immunology : CVI·Monique da Rocha Queiroz LimaFlavia Barreto dos Santos
Oct 27, 2012·Clinical and Vaccine Immunology : CVI·Alvina Clara FelixJosé Eduardo Levi

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Methods Mentioned

BETA
antigen capture
ELISA
PCR
electrophoresis

Software Mentioned

Excel
Panbio

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