Comparison of three commercially available serologic assays used to detect human parvovirus B19-specific immunoglobulin M (IgM) and IgG antibodies in sera of pregnant women

Journal of Clinical Microbiology
Allyson R Butchko, Jeanne A Jordan

Abstract

A split-sample study was conducted to evaluate the performances of three enzyme immunoassays (EIAs) utilizing one or more conformational antigens to detect human parvovirus B19 (B19V)-specific immunoglobulin M (IgM) or IgG in the sera of 198 pregnant women. We compared EIAs available from Biotrin International, Inc. (Dublin, Ireland), Medac Diagnostika (Wedel, Germany), and Mikrogen (Martinsried, Germany). Specimens with discordant results were analyzed further using an immunofluorescence assay (Biotrin). Equivocal data accounted for close to half of all the discrepant results for both IgM and IgG, with 7 of 15 discrepant results from the Medac and Mikrogen kits involving equivocal data and the Biotrin kit giving a single equivocal result. For each specimen, a consensus was established from the four test results if agreement occurred among at least three of four results. Overall, the highest percentage of agreement with the consensus results was seen when Biotrin kits were used; 194 (100%) of 194 and 194 (99.5%) of 195 results for IgM and IgG, respectively, agreed with the consensus results. When Medac kits were used, 189 (97.4%) of 194 and 191 (97.9%) of 195 results for IgM and IgG, respectively, agreed with the consensus, and...Continue Reading

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Citations

Jan 25, 2013·BMC Research Notes·Michelle Teodoro AlvesKarina Braga Gomes
Dec 20, 2007·The American Journal of the Medical Sciences·Stelios F AssimakopoulosCharalambos Gogos
Jul 26, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Marinko DobecNorbert Satz
Aug 8, 2007·Transfusion Medicine·A Parsyan, D Candotti
Nov 3, 2010·BJOG : an International Journal of Obstetrics and Gynaecology·R F LamontR Romero
Jun 15, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Fernando de OryAntonio Fuertes
Jan 26, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·O EgbunaJ Taylor
Sep 1, 2007·Arthritis Research & Therapy·Benedikt WeissbrichHermann J Girschick
Nov 4, 2016·Clinical Microbiology Reviews·Jianming QiuNeal S Young
Nov 28, 2012·Indian Journal of Medical Microbiology·S A Zaki
Jun 24, 2016·Microbiology Spectrum·Marie Louise Landry
Aug 6, 2021·Diagnostic Microbiology and Infectious Disease·Tim Van den AbeeleElizaveta Padalko

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