Comparison of three different methods for the detection of circulating tumor cells in mice with lung metastasis

Oncology Reports
Weifeng XuLurong Zhang

Abstract

Circulating tumor cells (CTCs) represent the key step of cancer cell dissemination. The alteration of CTCs correlates with the treatment outcome and prognosis. To enrich and identify CTCs from billions of blood cells renders a very challenging task, which triggers development of several methods, including lysis of RBC plus negative or positive enrichment using antibodies, and filter membrane or spiral microfluidics to capture CTCs. To compare the advantages of different enrichment methods for CTCs, we utilized the 4T1 breast cancer cells transfected with both green fluorescent protein (GFP) and luciferase to trace CTCs in the experimental lung metastasis model. Three methods were used to detect CTCs at the same time: bioluminescence assay, smearing method, and membrane filter method. The in vivo alive mouse imaging was used to dynamically monitor the growth of lung metastases. The sensitivity and accuracy of three detection methods were compared side-by-side. Our results showed that 1) the sensitivity of bioluminescence assay was the highest, but there was no information of CTC morphology; 2) the smearing method and membrane filter method could observe the detail of CTC morphology, such as in single or in cluster, while their s...Continue Reading

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Citations

Jan 31, 2018·Thyroid : Official Journal of the American Thyroid Association·Carrie C LubitzSareh Parangi
Jul 25, 2019·Cell Death & Disease·Caterina IeranòStefania Scala
Feb 8, 2019·Proceedings of the National Academy of Sciences of the United States of America·Sejeong ShinSang-Oh Yoon
Jul 27, 2021·International Journal of Pharmaceutics·Niloufar Hosseini-GivMaryam M Matin

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Methods Mentioned

BETA
chips
flow cytometry
membrane filtration
bioluminescence
fluorescence imaging

Software Mentioned

Spectrum Living Image
GraphPad Prism6

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