Comparison of three different methods for effective introduction of platelet-rich plasma on PLGA woven mesh

Biomedical Materials
Ji-Hye LeeJeong Joon Yoo

Abstract

For successful tissue regeneration, effective cell delivery to defect site is very important. Various types of polymer biomaterials have been developed and applied for effective cell delivery. PLGA (poly lactic-co-glycolic acid), a synthetic polymer, is a commercially available and FDA approved material. Platelet-rich plasma (PRP) is an autologous growth factor cocktail containing various growth factors including PDGF, TGFβ-1 and BMPs, and has shown positive effects on cell behaviors. We hypothesized that PRP pretreatment on PLGA mesh using different methods would cause different patterns of platelet adhesion and stages which would modulate cell adhesion and proliferation on the PLGA mesh. In this study, we pretreated PRP on PLGA using three different methods including simple dripping (SD), dynamic oscillation (DO) and centrifugation (CE), then observed the amount of adhered platelets and their activation stage distribution. The highest amount of platelets was observed on CE mesh and calcium treated CE mesh. Moreover, calcium addition after PRP coating triggered dramatic activation of platelets which showed large and flat morphologies of platelets with rich fibrin networks. Human chondrocytes (hCs) and human bone marrow stromal...Continue Reading

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Citations

Aug 4, 2018·The American Journal of Sports Medicine·Cheng-Hao ZhangTing-Wu Qin
Jan 16, 2019·Macromolecular Bioscience·Sajeesh Kumar Madhurakkat PerikamanaHeungsoo Shin
Sep 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rifang LuoYunbing Wang

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