Comparison of three dispersion procedures for quantitative recovery of cultivable species of subgingival spirochetes.

Journal of Clinical Microbiology
S L SalvadorW J Loesche

Abstract

Spirochetes are usually the predominant organisms observed microscopically in subgingival plaques removed from tooth sites associated with periodontitis, but these organisms are rarely isolated by cultural means, presumably because the media do not support their growth and/or because these fragile organisms are disrupted by the various procedures used to disperse plaque samples. In the present investigation, three dispersal procedures, sonification, mechanical mixing, and homogenization, were compared for their ability to permit the isolation of Treponema denticola, Treponema vincentii, Treponema socranskii, and Treponema pectinovorum from plaque samples on media that support the growth of these species. Plaque samples in which the spirochetes averaged 50% of the microscopic count were chosen. The highest viable recoveries of spirochetes were observed when the plaques were dispersed with a Tekmar homogenizer, and the lowest occurred with sonification. The highest recoveries averaged only about 1% of the total cultivable counts, indicating either that the sought-after species were minor members of the flora or that the dispersal procedures were still too harsh. A total of 91% of the isolates were T. denticola, 5% were T. socrans...Continue Reading

Citations

Apr 1, 1990·Oral Microbiology and Immunology·M UmedaT Mitsuoka
Aug 1, 1994·Oral Microbiology and Immunology·S ShapiroB Guggenheim
Oct 1, 1995·Oral Microbiology and Immunology·T KosekiY Benno
Nov 1, 1988·Advances in Dental Research·W J Loesche
Apr 1, 1993·Oral Microbiology and Immunology·E C ChanA Klitorinos
Oct 1, 1994·Oral Microbiology and Immunology·Y S QiuE C Chan
Jan 11, 2020·Frontiers in Cellular and Infection Microbiology·Hong Min NgEric C Reynolds
Jun 1, 1990·Journal of Clinical Microbiology·L A MagnarelliG R Riviere
Oct 1, 1988·Journal of Clinical Microbiology·S A SyedW J Loesche
May 9, 1998·Journal of Clinical Microbiology·A MoterU B Göbel

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