PMID: 20650078Apr 1, 1995Paper

Comparison of three in vitro cytotoxicity assays for estimating surfactant ocular irritation

Toxicology in Vitro : an International Journal Published in Association with BIBRA
L VianJ P Cano

Abstract

Three in vitro cytotoxicity assays [neutral red uptake assay (NRU), MTT test and total protein content determination (TPC)] were analysed to assess their value for predicting the ocular irritancy potential of 20 surfactants. For each test, three established cell lines (SIRC rabbit corneal cells, Balb/c 3T3 and L929 mouse fibroblasts) were used. The concentration that induced 50% inhibition relative to controls (IC(50)) was calculated for each test, cell line and chemical. In vivo ocular irritancy data were compared with in vitro results. None of these assays provided a marked correlation of surfactant ocular irritation (Spearman or Pearson correlation coefficient lower than 0.65, P < 0.01 and moderate agreement with Kappa test). An IC(50) threshold of 700 mug/ml was set to discriminate between surfactant irritation and non-irritation. Three compounds were detected as false negatives (CHAPS, CHAPSO and sodium taurocholate), and two as false positives (Triton X155 and Brij 35).

References

May 1, 1992·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·J F SinaP D Gautheron
Jul 1, 1991·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·L H BrunerR D Parker
Feb 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C ShopsisD M Stark
Feb 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H North-RootJ E Heinze
Oct 1, 1992·International Journal of Cosmetic Science·M Balls
Oct 1, 1989·International Journal of Cosmetic Science·P TachonM Prunieras
Jan 1, 1988·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E BorenfreundN Martin-Alguacil
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·O BleinK G Dossou
Mar 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M CornelisJ Wepierre
Jul 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D M BagleyA P Walker
Mar 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T HusøyJ Jenssen

❮ Previous
Next ❯

Citations

Nov 9, 2012·Pharmaceutical Research·Jaclyn M HosmerLuciana B Lopes
Mar 24, 2004·Toxicon : Official Journal of the International Society on Toxinology·B JiangL J An
Jun 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K ChibaK Tohyama
Feb 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K ChibaY Ohno
May 11, 2013·Journal of Asian Natural Products Research·Zhen-Chang ZhangXiao-Dong Xie
Apr 22, 2008·Neurological Research·Bo JiangXiu Li Zhang
Nov 12, 2015·Drug Development and Industrial Pharmacy·Mária Budai-SzűcsErzsébet Csányi
Jul 16, 2014·Drug Development and Industrial Pharmacy·Anna Czajkowska-KośnikMałgorzata Sznitowska
Oct 17, 2006·Cutaneous and Ocular Toxicology·Ann Wright, Mary Mowrey-McKee
Jan 19, 2016·Journal of Pharmacological and Toxicological Methods·Daniela S Masson-MeyersChukuka S Enwemeka
Mar 25, 2016·European Journal of Dentistry·La-Ongthong Vajrabhaya, Suwanna Korsuwannawong
Dec 4, 2012·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Elena RavazzoloPaolo Caliceti
Mar 2, 2010·Chemico-biological Interactions·Xian-Ju HuangZong-Hui Yuan
Jan 1, 2009·Environmental Toxicology and Pharmacology·Peng ZhangLiji Jin
Oct 15, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L J AnB Jiang
Oct 13, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nathalie DijouxPierre-Jacques Ferret
Sep 5, 2014·Journal of the Science of Food and Agriculture·Joanna RychlikWłodzimierz Grajek
Jun 4, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Dong-Joo KwonJinseu Park
May 4, 2016·Colloids and Surfaces. B, Biointerfaces·Biao LuIsabelle Pezron
Apr 28, 2009·Biomaterials·Raquel De SouzaMicheline Piquette-Miller
Jan 11, 2013·International Journal of Toxicology·Lillian C BeckerF Alan Andersen
Jun 29, 2005·International Journal of Toxicology·UNKNOWN Cosmetic Ingredient Review Expert Panel
Nov 27, 2018·Pharmaceutical Development and Technology·Mohammad Mehdi MahboobianIlva Dana Rupenthal
Oct 27, 2018·Journal of Veterinary Research·Sylwia TobólskaAndrzej Krzysztof Siwicki
Dec 5, 2012·Dental Materials Journal·Hiroshi KanoHiroshi Murata
Jun 9, 2004·International Journal of Occupational Safety and Ergonomics : JOSE·Jolanta Skowroń, Lidia Zapór
Aug 4, 2010·Journal of Natural Products·Xin-Gang DuDe-An Guo
Jul 19, 2018·International Journal of Pharmaceutics·Maria Teresa Junqueira GarciaLuciana Biagini Lopes
Apr 11, 2019·Ophthalmic Research·Anna Matysik-WoźniakRobert Rejdak

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Toxicology in Vitro : an International Journal Published in Association with BIBRA
H ItagakiM Umeda
Toxicology in Vitro : an International Journal Published in Association with BIBRA
P CatrouxM Cottin
Journal of Asian Natural Products Research
Li WangZheng-Tao Wang
© 2021 Meta ULC. All rights reserved