Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
Donald C SheppardScott G Filler

Abstract

Quantitative culture, quantitative PCR and the galactomannan enzyme immunoassay (EIA) were compared for their ability to determine the pulmonary fungal burden in a murine model of invasive aspergillosis. Quantitative culture of specimens containing hyphae under-represented the absolute fungal burden in established infection when compared with the two other methods. The best correlation was observed between the two non-culture methods. Higher variability was observed with the galactomannan EIA when compared with quantitative PCR. Collectively, these data suggest that quantitative PCR is the preferred method for determination of the pulmonary fungal burden in experimental aspergillosis.

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Citations

Jul 3, 2013·Immunobiology·Amal Atia Mhfuod El-MuzghiMilena Kataranovski
Jul 26, 2008·Medical Mycology·K V Clemons, D A Stevens
Jan 18, 2008·The Journal of Infectious Diseases·Sara SpikesGregory S May
Sep 29, 2006·Antimicrobial Agents and Chemotherapy·Donald C SheppardScott G Filler
Aug 3, 2010·Eukaryotic Cell·Daniele E EjzykowiczScott G Filler
May 5, 2010·Infection and Immunity·Nadia Al-BaderDonald C Sheppard
Nov 28, 2007·Infection and Immunity·Camille BretzKieren A Marr
Mar 6, 2009·Journal of Clinical Microbiology·M L HerreraB L Wickes
Jun 27, 2013·Indian Journal of Pharmaceutical Sciences·P S KalaskarM M Gatne
Apr 9, 2014·Journal of Immunological Methods·Tobias M Hohl
Feb 18, 2009·Molecular Microbiology·Daniele E EjzykowiczScott G Filler
Nov 11, 2010·Molecular Microbiology·Hong LiuScott G Filler
Nov 6, 2009·Cellular Microbiology·Fabrice N GravelatDonald C Sheppard
Jun 3, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Matthias Brock
Jul 3, 2013·American Journal of Rhinology & Allergy·Fang ZhangChangqing Zhao
Nov 8, 2018·The Journal of Antimicrobial Chemotherapy·Rogelio de J Treviño-RangelGloria M González
Nov 1, 2006·Current Opinion in Infectious Diseases·Johan MaertensKoen Theunissen
Sep 16, 2017·The Journal of Infectious Diseases·Russell E Lewis, Paul E Verweij
Feb 26, 2019·Frontiers in Immunology·Marco BlickensdorfMarc Thilo Figge
Apr 9, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Melissa A GessnerChad Steele
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jessica L WernerChad Steele
Apr 20, 2018·Frontiers in Microbiology·Toine MercierJohan Maertens

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Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.