Comparison of three new nonculture tests in the diagnosis of Chlamydia genital infections

Journal of Adolescent Health Care : Official Publication of the Society for Adolescent Medicine
H SchubinerB Hershman

Abstract

This study compares three new rapid nonculture tests to cell culture with passage for the diagnosis of Chlamydia genital infections in sexually active adolescent and young adult females. Two hundred consecutive patients having a pelvic examination had cervical samples taken for the following: Papanicolaou smear, gonorrhea culture, Chlamydia cell culture, direct fluorescent antibody (DFA; Difco), isotopic DNA probe (Gen-Probe), and enzyme immunoassay (EIA; IDEIA III, Novo BioLabs). After resolution of discrepant results, 25 of the specimens were judged to be positive. The DFA identified 17 of the 25, with 3 false-positive results; the DNA probe identified 20 of the 25, with no false positive results; and the EIA identified 22 of the 25, with one false-positive result. The sensitivities, specificities, and positive and negative predictive values, respectively, were DFA, 68%, 98.2%, 85%, 95.5%; DNA probe, 80%, 100%, 100%, 97.2%; and EIA, 88%, 99.4%, 95.6%, 98.3%. These new rapid nonculture tests are comparable and relatively reliable, with trends favoring the EIA and the DNA probe. Further studies with larger samples are needed to determine their clinical utility.

References

Sep 1, 1986·Diagnostic Microbiology and Infectious Disease·R W RyanR C Tilton

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Citations

Jun 1, 1995·Pediatric Annals·M R Chacko, W D Rosenfeld
Dec 6, 2002·Journal of Medical Microbiology·Emma J WatsonBabil Stray Pederson
Jul 19, 1993·The Medical Journal of Australia·S M GarlandJ A McInnes

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