Comparison of three non-culture techniques for detection of Chlamydia trachomatis in genital tract specimens

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
C J Hall, C Nelder

Abstract

A direct immunofluorescence (DIF) technique (Imagen) and two enzyme immunoassay (EIA) techniques (Chlamydiazyme and IDEIA) were compared for the detection of Chlamydia trachomatis in genital specimens from 502 attenders at a genitourinary medicine clinic. Eighty-two attenders were regarded as infected: 67 with positive results by at least two of the three techniques and 15 by virtue of elementary bodies detected in stored EIA buffer samples. With a positivity criterion of greater than or equal to 6 bodies Imagen was 76% sensitive for men and 61% sensitive for women. The sensitivity of Chlamydiazyme was 73% for men and 90% for women; comparative values for IDEIA were 80% and 71%, respectively. All three techniques were over 98% specific. Sampling order appeared to influence the sensitivity of IDEIA for specimens from men. All three techniques were less sensitive in the absence of cervicitis. The performances of the EIA techniques compared favourably with that of the more established technique of DIF.

References

Apr 1, 1988·Diagnostic Microbiology and Infectious Disease·E WeismeierL Manduke
Feb 1, 1986·Journal of Clinical Pathology·P Morgan-CapnerA Saeed
Jan 1, 1986·Journal of Clinical Microbiology·E S LipkinJ Schachter
Feb 1, 1986·Journal of Clinical Microbiology·L V HowardJ E Herrmann
Apr 1, 1986·Postgraduate Medical Journal·G L Ridgway
Jun 1, 1986·Genitourinary Medicine·K C MohantyM H Hambling
Sep 1, 1986·American Journal of Clinical Pathology·R A Levy, A L Warford
Oct 1, 1986·European Journal of Clinical Microbiology·L GrillnerH Hammar
Oct 1, 1986·European Journal of Clinical Microbiology·P Pothier, A Kazmierczak
Jun 1, 1986·Journal of Clinical Microbiology·B A ForbesL Welych
Dec 1, 1986·European Journal of Clinical Microbiology·A H van der WilligenE Stolz
Dec 1, 1985·European Journal of Clinical Microbiology·K H TjiamM F Michel
Aug 1, 1985·European Journal of Clinical Microbiology·J van UlsenE Stolz
Apr 1, 1985·Sexually Transmitted Diseases·E M DunlopR Woodland
Aug 1, 1984·The British Journal of Venereal Diseases·K SyrjänenS Saarikoski
May 3, 1984·The New England Journal of Medicine·M R TamR C Nowinski
Dec 1, 1984·The British Journal of Venereal Diseases·P E MundayD Taylor-Robinson

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Citations

Nov 1, 1994·Zhurnal mikrobiologii, epidemiologii, i immunobiologii·M E TrofimovaS V Shubin
Dec 1, 1990·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H C ClaasW G Quint
Aug 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V Neman-SimhaC Bébéar
Jan 1, 1996·Microbiology and Immunology·I TakashimaN Hashimoto
Jun 1, 1991·Genitourinary Medicine·D Taylor-Robinson, B J Thomas
Apr 20, 2002·Arquivos de neuro-psiquiatria·Jacqueline Nelisis ZanoniMarcílio Hubner de Miranda Neto
Nov 13, 2003·Physical Review Letters·Michael R ShirtsVijay S Pande
Dec 24, 2018·Expert Review of Molecular Diagnostics·Robert DuncanBryan Lanning

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