Comparison of three nucleic acid amplification tests for detection of Chlamydia trachomatis in urine specimens

Journal of Clinical Microbiology
Charlotte A GaydosThomas C Quinn

Abstract

Traditionally, culture and immunoassays have been performed for the detection of sexually transmitted diseases, including Chlamydia trachomatis. However, these assays may often require invasive specimen collection methods, such as female cervical and male urethral swabs. Recently, nucleic acid amplification tests (NAATs) have been approved for testing for the presence of C. trachomatis in urine samples. Our objective was to compare the sensitivities and specificities of C. trachomatis detection in urine samples with three NAATs: the Abbott LCx (LCx), BD ProbeTec ET (ProbeTec), and Gen-Probe APTIMA Combo 2 (AC2). Urine specimens (n = 506) were collected from both symptomatic and asymptomatic males and females from various high school health clinics. Specimens were tested for C. trachomatis with the three NAATs, and a true-positive result was defined as any two positive NAATs. The C. trachomatis prevalence was 14.8% (75 of 506 samples). Of the 75 urine samples defined as true positives, LCx detected 72, ProbeTec 72, and AC2 detected 75. The sensitivities of LCx, ProbeTec, and AC2 for C. trachomatis detection were 96.0, 96.0, and 100%, and the specificities were 99.1, 100, and 98.8%, respectively. Four of five samples that were po...Continue Reading

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Citations

Dec 21, 2006·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·T Meyer
Dec 14, 2006·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·V BremerO Hamouda
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Nov 11, 2010·Sexual Health·Shailendra SawleshwarkarAdrian Mindel
Mar 1, 2006·Sexually Transmitted Diseases·Charlotte Ann GaydosJulia Ann Schillinger
Apr 7, 2007·Sexually Transmitted Diseases·Haleta E LimaFátima Soares Motta Noronha
May 21, 2008·Sexually Transmitted Diseases·Mary-Ann B ShaferJulius Schachter
May 2, 2008·Sexually Transmitted Diseases·Charlotte A GaydosJohn Papp
Nov 29, 2012·Pediatric Emergency Care·Monika GoyalCynthia Mollen
Jan 23, 2013·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Luc BéhanzinMichel Alary
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