Comparison of three PCR methods for detection of Helicobacter pylori DNA and detection of cagA gene in gastric biopsy specimens

World Journal of Gastroenterology : WJG
S-I SmithA-O Coker

Abstract

To comparatively evaluate PCR and other diagnostic methods (the rapid urease test and / or culture) in order to determine which of the three PCR methods (ureA, glmM and 26-kDa, SSA gene) was most appropriate in the diagnosis of Helicobacter pylori (H pylori ) infection and also to evaluate the detection of a putative virulence marker of H pylori, the cagA gene, by PCR in biopsy specimens. One hundred and eighty-nine biopsy specimens were collected from 63 patients (three biopsies each) undergoing upper gastroduodenal endoscopy for various dyspeptic symptoms. The PCR methods used to detect H pylori DNA directly from biopsies were the glmM, 26-kDa, ureA and then cagA was used to compare the culture technique and CLO for urease with the culture technique being used as the gold standard. Thirty-five percent of the biopsies were positive for H pylori DNA using the 3 PCR methods, while 68% of these were positive for the cagA gene. Twenty-four percent of the biopsies were negative for H pylori DNA in all PCR methods screened. The remaining 41% were either positive for ureA gene only, glmM only, 26-kDa only, or ureA + glmM, ureA + 26-kDa, glmM + 26-kDa. Out of the 35% positive biopsies, 41% and 82% were positive by culture and CLO resp...Continue Reading

Citations

Jul 28, 2009·Diagnostic Microbiology and Infectious Disease·Lyudmila BoyanovaIvan Mitov
Dec 1, 2015·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Lyudmila BoyanovaIvan Mitov
Nov 30, 2005·Archives of Medical Research·Jaime Raúl Zúñiga-NoriegaElvira Garza-González
Jan 10, 2012·BMC Gastroenterology·Javed YakoobWasim Jafri
Sep 7, 2012·Diagnostic Microbiology and Infectious Disease·Daniel YordanovIvan Mitov
Mar 7, 2012·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Monica ContrerasFabián Michelangeli
Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·S Mendoza-ElizaldeN Velázquez-Guadarrama
Nov 18, 2004·Annals of Clinical Microbiology and Antimicrobials·Raul V DesturaRichard L Guerrant
Jun 15, 2010·Cancer Epidemiology·Angela Rosa AndréSílvia Helena Barem Rabenhorst
Jan 29, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Valeska Portela LimaSílvia Helena Barem Rabenhorst
Dec 7, 2016·British Journal of Biomedical Science·Najmiyatul FadilahMd Mostafizur Rahman
Feb 10, 2009·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Tanya FancyHassan H Ramadan
May 6, 2014·Journal of Pediatric Gastroenterology and Nutrition·Hamza KarabiberAli Ozer
Aug 21, 2009·Digestive Diseases and Sciences·Tesiê L FonsecaPedro E A Silva
Jan 26, 2006·The American Journal of Surgical Pathology·Veronika ZsiklaGieri Cathomas
Jun 28, 2011·Antonie van Leeuwenhoek·Lyudmila BoyanovaIvan Mitov
Aug 12, 2014·World Journal of Gastroenterology : WJG·Isabel AdlerValeria Denninghoff
Jul 20, 2019·Journal of Environmental Health Science & Engineering·Marzieh FarhadkhaniBahram Nikmanesh
Nov 24, 2016·Frontiers in Microbiology·Sandra Mendoza-ElizaldeNorma Velázquez-Guadarrama
Jan 9, 2009·Journal of Clinical Microbiology·Mitsushige SugimotoDavid Y Graham

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