Comparison of three preparatory methods for detection of bacteremia by MALDI-TOF mass spectrometry

Diagnostic Microbiology and Infectious Disease
Ryan T SaffertRobin Patel

Abstract

We evaluated 3 preparatory methods for processing positive blood culture bottle broths prior to matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis-differential centrifugation, 10% sodium dodecyl sulfate (SDS), and the Sepsityper kit. Initial evaluation used genus and species level cutoff scores of 1.700-1.999 and ≥ 2.000, respectively. Processing of an initial 79 blood bottle cultures by differential centrifugation yielded correct identifications of 51 (65%) to the genus and 34 (43%) to the species levels. One hundred and one different blood bottles were simultaneously processed with 10% SDS and Sepsityper. Both yielded genus-level identifications for 77 (76%), and the 2 yielded species-level identifications for 49 (49%) and 54 (54%) of bottles, respectively. Adjustment of the score cutoff criteria for genus (1.500-1.699) and species (≥ 1.700) improved identification percentages, particularly for species-level identifications (P < 0.05).

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Citations

Apr 19, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Robin Patel
Dec 12, 2012·Enfermedades infecciosas y microbiología clínica·Yanick Hoyos-MallecotJosé María Navarro-Marí
Oct 30, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ritu BanerjeeRobin Patel
Nov 17, 2017·Clinical Microbiology Reviews·Poornima RamananRobin Patel
Jul 31, 2013·American Journal of Clinical Pathology·Christina Wojewoda, UNKNOWN Education Committee of the Academy of Clinical Laboratory Physicians and Scientists
May 9, 2014·Journal of Clinical Microbiology·Raquel M MartinezSusan M Butler-Wu
Oct 30, 2018·Journal of Medical Microbiology·Jonathan ZintgraffMauricio Santos
Dec 1, 2017·Critical Reviews in Microbiology·Walter FlorioAntonella Lupetti
Feb 8, 2019·Infectious Diseases·Helena EnrothAnna-Karin Pernestig
Dec 29, 2020·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Mohammed S AlmuhayawiVolkan Özenci

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