Comparison of three rapid detection systems for type A influenza virus on tracheal swabs of experimentally and naturally infected birds

Avian Pathology : Journal of the W.V.P.A
G CattoliI Capua

Abstract

The present paper reports of the comparison between three rapid virus detection systems and virus isolation (VI) from pooled tracheal swabs collected from naturally and experimentally infected birds with a low pathogenicity avian influenza virus of the H7N3 subtype. The relative sensitivity, specificity and agreement (K value) were calculated for a commercial antigen capture enzyme immunoassay (AC-EIA) and for two nucleic acid detection tests, a one-step reverse transcriptase-polymerase chain reaction (RT-PCR) and a real-time RT-PCR (RRT-PCR), both targeting the M gene. The results indicate that in experimentally infected turkeys VI was positive from the pooled tracheal swabs collected from day 3 to day 10. One-step RT-PCR was able to detect influenza RNA from samples collected from day 3 to day 12, while RRT-PCR amplified influenza RNA in swabs collected from day 3 to day 15. The AC-EIA test yielded positive results between day 5 and day 10 post-infection. On field samples, the K value between the AC-EIA and VI tests was 0.82. Compared with VI, the relative sensitivity of this test was 88.9% (CI95 = 85.2-92.6) and the relative specificity was 95.7% (CI95 = 93.7-97.7). The K value between the RT-PCR and VI tests was 0.88. Compa...Continue Reading

References

May 1, 1992·Journal of Clinical Microbiology·K A Ryan-PoirierY Kawaoka
Sep 17, 1994·The Veterinary Record·T M ChambersK L Watkins
Mar 14, 1998·Virus Research·M GarcíaM L Perdue
Jun 22, 2000·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·E StarickO Werner
Oct 25, 2003·Avian Diseases·Karen DybkaerPoul H Jørgensen
Oct 25, 2003·Avian Diseases·E Starick, O Werner
Jul 24, 2007·Proceedings. Biological Sciences·James TruscottAzra C Ghani
Jul 1, 1981·Avian Pathology : Journal of the W.V.P.A·D J Alexander, D Spackman
Aug 1, 2000·Avian Pathology : Journal of the W.V.P.A·I Capua, S Marangon

❮ Previous
Next ❯

Citations

Jun 7, 2006·Avian Pathology : Journal of the W.V.P.A·Ilaria Capua, Dennis J Alexander
Sep 23, 2006·Avian Pathology : Journal of the W.V.P.A·Maria Serena BeatoIlaria Capua
Jul 11, 2007·Avian Pathology : Journal of the W.V.P.A·Calogero TerreginoIlaria Capua
Apr 7, 2006·Journal of Clinical Microbiology·Daniel P FedorkoKanta Subbarao
Jul 1, 2009·Journal of Molecular and Genetic Medicine : an International Journal of Biomedical Research·Najmeh MoslehAli Mohammadi
Sep 12, 2013·Future Microbiology·Mohamed E El ZowalatyHossam M Ashour
Feb 3, 2007·Expert Review of Anti-infective Therapy·Guy Vernet
Jul 1, 2007·Influenza and Other Respiratory Viruses·Luke T DaumJames P Chambers
Aug 19, 2008·Zoonoses and Public Health·E Couacy-HymannG Cattoli
May 1, 2007·Influenza and Other Respiratory Viruses·Eugeny E FesenkoAlexander S Zasedatelev
Jan 19, 2008·Zoonoses and Public Health·G Cattoli, C Terregino
Jan 19, 2008·Zoonoses and Public Health·D J Alexander
Jul 27, 2010·Journal of Virological Methods·Robert B MandellRamon Flick
May 15, 2007·Avian Diseases·David L SuarezElizabeth Ellis
Mar 11, 2008·Applied and Environmental Microbiology·H SchönenbrücherM Bülte
Mar 16, 2005·The Veterinary Record·C TerreginoM Scremin
Sep 2, 2017·Frontiers in Microbiology·Guanliu YuYouxiang Diao
Sep 1, 2016·BMC Research Notes·Agustiningsih AgustiningsihDavid Handojo Muljono

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.