PMID: 6834827Apr 1, 1983

Comparison of trans-stilbene oxide, phenobarbital and 3-methylcholanthrene as inducers of steroid metabolism by the rat liver microsomal cytochrome P-450 system

Journal of Steroid Biochemistry
J Meijer, J W DePierre

Abstract

The metabolism of testosterone and androstenedione by liver microsomes was investigated after treatment of rats with trans-stilbene oxide, phenobarbital, or 3-methylcholanthrene. Conditions for linearity of the assay with time and amount of cytochrome P-450, as well as saturating substrate concentrations, were established. The metabolites were separated by thin-layer chromatography and quantitated by scintillation counting. The rates of formation of different testosterone and androstenedione metabolites after induction with trans-stilbene oxide or phenobarbital were similar, indicating that these xenobiotics induce the same isozyme of cytochrome P-450. This conclusion was further supported using inhibitors of cytochrome P-450 (SKF-525A, metyrapone and alpha-naphthoflavone) and with immunoinhibition by antibodies directed towards the phenobarbital-inducible form of cytochrome P-450. After treatment with trans-stilbene oxide or phenobarbital, the specific rates of formation of the 6 beta- and/or 2 beta-hydroxy metabolites and of 17 beta-hydroxy-4-androstene-3,16-dione were increased. In contrast, administration of 3-methylcholanthrene led to decreases in the specific rates of formation of almost all testosterone and androstenedio...Continue Reading

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K S KorachJ A McLachlan
Apr 6, 1978·Biochimica Et Biophysica Acta·J W DePierre, L Ernster
Mar 1, 1979·Toxicology·G FeuerF A De la Iglesia
Jan 1, 1976·Neuroendocrinology·J Fishman
Nov 1, 1976·Obstetrical & Gynecological Survey·J E Gunning
Jan 1, 1966·Advances in Enzyme Regulation·R KuntzmanA H Conney
Apr 29, 1980·Biochemical and Biophysical Research Communications·Y Nakamura, S Ueda

Citations

Jan 1, 1987·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·M J RonisD Peakall
Feb 1, 1990·American Journal of Obstetrics and Gynecology·J A BaronF Levi
Jun 1, 1990·Biochemical Pharmacology·A BlanckA Rane
Jun 1, 1993·Journal of Biochemical Toxicology· Fuchs SYuG A Belitsky
Aug 1, 1987·Epidemiology and Infection·R E Hope-Simpson, D B Golubev
May 1, 1996·Environmental Health Perspectives·H Bartsch, E Hietanen
May 13, 2014·The Journal of Pharmacology and Experimental Therapeutics·John R Cashman, Marc R Azar

Related Concepts

alpha-naphthoflavone
Stilbene oxide, trans-(+-)-isomer
Androstenedione
Benzoflavones
Cytochrome P-450 Oxygenase
Hydroxylation
Methylcholanthrene
SU 4885
Microsomes, Liver
Luminal

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