Comparison of two assay procedures for lignin peroxidase

Enzyme and Microbial Technology
D S. Arora, P K. Gill

Abstract

The most widely accepted assay for detecting lignin peroxidase, based on the oxidation of veratryl alcohol to veratraldehyde, suffers from some drawbacks. At 310 nm, the wavelength at which the assay is performed, some other materials like lignins, quinonic compounds and aromatics also exhibit strong absorbance thus interfering with the estimation when present in the media. The present study reports the lignin peroxidase production by some white rot fungi under different nutritional conditions. The veratryl alcohol oxidation assay procedure for lignin peroxidase has been compared with another method based on the oxidation of the dye azure B involving absorbance measurements in the visible range. The latter method proved to be much more advantageous over the veratryl alcohol oxidation method, in media supplemented with malt extract, lignin preparations and agricultural residues. The enzyme production by veratryl alcohol assay could be detected only in mineral salts broth. By the azure B assay the enzyme activity was detected in all the media tested. The supplements gave varied response in different media. Veratryl alcohol enhanced the enzyme production in malt extract broth and mineral salts malt extract broth. Among the lignin ...Continue Reading

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Citations

Sep 24, 2008·Applied Microbiology and Biotechnology·Deepak Singh, Shulin Chen
Apr 11, 2012·Folia Microbiologica·Stanislav ObrucaAndrea Lichnova
Apr 20, 2010·Journal of Environmental Sciences (China)·Tony Hadibarata, Sanro Tachibana
Dec 24, 2009·Canadian Journal of Microbiology·Paramjit Kaur Bajwa, Daljit Singh Arora
Aug 1, 2007·Pakistan Journal of Biological Sciences : PJBS·Tony HadibarataKazutaka Itoh
Jul 20, 2014·Environmental Science and Pollution Research International·Yang LiuYibin He
Dec 12, 2013·Environmental Science and Pollution Research International·Chin Hong NeohZaharah Ibrahim
Oct 15, 2011·BMC Biotechnology·Luaine BandounasHarald J Ruijssenaars
Jun 23, 2007·Bioresource Technology·E M SilvaA M F Milagres
Jan 3, 2012·Bioresource Technology·Tony Hadibarata, Risky Ayu Kristanti
Sep 22, 2016·SpringerPlus·Prashant D KunjadiaGaurav S Dave
Apr 4, 2017·International Journal of Environmental Research and Public Health·Dianny Silva LisboaCristina M Souza-Motta
Jun 16, 2014·Applied Biochemistry and Biotechnology·Gupteshwar GuptaSaroj Mishra
Jun 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jaturong KumlaSaisamorn Lumyong
Mar 1, 2015·Applied Biochemistry and Biotechnology·Mohamed TahaEric M Adetutu
Jun 20, 2018·Microbial Ecology·Lia Costa Pinto WentzelLara Durães Sette
Mar 9, 2019·Environmental Science and Pollution Research International·Maria R S VasconcelosLara D Sette
Jul 13, 2018·Mycobiology·Satadru Pramanik, Sujata Chaudhuri
Feb 13, 2021·International Journal of Biological Macromolecules·Anil Kumar SinghAbhay Raj
Jul 30, 2020·Environmental Science and Pollution Research International·Xiaoxue ZhangZhixin Yang

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