Comparison of two automated DNA amplification systems with a manual one-tube nested PCR assay for diagnosis of pulmonary tuberculosis.

Journal of Clinical Microbiology
K Y YuenW H Seto

Abstract

Eighty-four specimens of respiratory secretions culture positive for mycobacteria (70 positive for Mycobacterium tuberculosis and 14 positive for nontuberculous mycobacteria) and 120 culture-negative specimens were evaluated by three DNA amplification techniques: a manual in-house single-tube nested PCR (nPCR) and two commercial automated assays (the Cobas Amplicor System [aPCR-h] from Roche Diagnostic Systems and the Abbott LCx Probe System [aLCx-p] from Abbott Laboratories). The overall diagnostic sensitivities of the nPCR, aPCR-h, and aLCx-p were 77.1, 84.3, and 77.1%, respectively, and the sensitivities were 57.9, 57.9, and 36.8%, respectively, for smear-negative specimens. Specimens culture positive for nontuberculous mycobacteria were negative by all three assays. Eight culture-negative specimens which were positive by one or more assays had previously been documented by culture to be positive for M. tuberculosis and were taken from patients who were treated with antituberculosis agents. Retesting of specimens negative by one assay by the other two assays revealed that each test had its unique group of negative specimens. When considering the DNA extraction and amplification steps of these assays separately, it was found ...Continue Reading

References

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Citations

Jan 20, 2006·FEMS Immunology and Medical Microbiology·Aysen BayramIclal Balci
Aug 5, 2009·Molecular Diagnosis & Therapy·Seetha V BalasinghamTone Tønjum
Jun 14, 2012·Journal of Clinical Microbiology·Enrico TortoliDaniela M Cirillo
Jun 14, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J H K ChenW-C Yam
Aug 14, 2020·International Journal of Molecular Sciences·Cyril Chik-Yan YipKelvin Kai-Wang To
Apr 6, 2006·Journal of Medical Microbiology·Wing-Cheong YamPak-Leung Ho
Jul 20, 2016·Tuberculosis and Respiratory Diseases·Hamed Ebrahimzadeh LeylabadloMohammad Asgharzadeh
Dec 20, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sonia H MontenegroRichard A Oberhelman
Nov 12, 2005·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·V C C ChengK Y Yuen
Jul 5, 2003·Journal of Clinical Microbiology·Olga L SarmientoWilliam C Miller
Dec 17, 1997·Journal of Clinical Microbiology·J P CegielskiL B Reller
Mar 30, 2002·Journal of Clinical Microbiology·Patrick C Y WooKwok-Yung Yuen
Mar 4, 2000·Journal of Clinical Microbiology·A García-QuintanillaM T Jiménez de Anta

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