Comparison of two chromogenic media and evaluation of two molecular based identification systems for Enterobacter sakazakii detection

BMC Microbiology
A LehnerRoger Stephan

Abstract

Enterobacter sakazakii is a foodborne pathogen that has been associated with sporadic cases and outbreaks causing meningitis, necrotizing enterocolitis and sepsis especially in neonates. The current FDA detection method includes two enrichment steps, the subculturing of the second enrichment broth on a selective agar (VRBG), a further subculturing of selected grown colonies on TSA and the subsequent biochemical identification of yellow-pigmented colonies by API20E. However, there is a strong need for simplified methods for isolation and identification of E. sakazakii. In this study, two chromogenic media, which allow to indicate presumptive E. sakazakii colonies by the alpha glucosidase activity, as well as a newly developed 1,6-alpha-glucosidase based conventional PCR assay and a rRNA oligonucleotide probe based commercial test system for identification of presumptive E. sakazakii were evaluated on 98 target and non-target strains. The methods were compared with respect to specificity aspects. A total of 75 presumptive E. sakazakii and 23 non-target strains were analysed by using chromogenic media, alpha-glucosidase based PCR assay, and the VIT assay. For most presumptive E. sakazakii strains on the chromogenic media, the PCR ...Continue Reading

References

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Citations

May 10, 2008·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·M Friedemann
Dec 5, 2009·Foodborne Pathogens and Disease·Brendan HealySéamus Fanning
Jul 26, 2013·Foodborne Pathogens and Disease·Xiaohui DongWeipeng Guo
Oct 31, 2006·Applied and Environmental Microbiology·Carol Iversen, Stephen J Forsythe
Mar 4, 2008·Applied and Environmental Microbiology·Carol IversenRoger Stephan
Sep 21, 2007·Journal of Clinical Microbiology·Carol IversenHan Joosten
Feb 28, 2015·Biological Research·Md FakruddinMd Hoque
Dec 23, 2017·Antonie van Leeuwenhoek·Veronika KadlicekovaHana Drahovska
Dec 25, 2008·Yonsei Medical Journal·Jung Beom KimHiun Suk Chae
Mar 14, 2012·Journal of Food Protection·Sarah NorbergPaul D Cotter
Mar 30, 2007·International Journal of Systematic and Evolutionary Microbiology·Roger StephanAngelika Lehner
Apr 28, 2016·World Journal of Microbiology & Biotechnology·Wanyi ChenZhenmin Liu
Jan 12, 2011·Journal of Food Protection·Bernd HeidenreichManfred Gareis
Oct 6, 2017·Frontiers in Microbiology·Eva J ScharingerKristina Schauer

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Datasets Mentioned

BETA
AM075208
DQ273680
DQ273681
DQ273682
DQ273683
DQ273684
DQ273685
DQ273686
DQ273687
DQ273688

Methods Mentioned

BETA
PCR
ribotyping
electrophoresis

Software Mentioned

ARB
TREEPUZZLE

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