Comparison of two chromogenic media for the detection of Listeria monocytogenes with the plating media recommended by EN/DIN 11290-1

International Journal of Food Microbiology
Biserka BeckerWilhelm H Holzapfel

Abstract

The efficacy of two selective chromogenic culture media, Agar Listeria Ottaviani and Agosti (ALOA) and RAPID' L. mono for the detection of Listeria monocytogenes in food, was compared with that of an official culture method according to the EN/DIN 11290-01 and -02 protocols [corresponding to the section 35 LMBG (German Food Act) method]. A total of 310 pre-packed ready-to-eat food samples (100 of graved and cold smoked salmon, 130 of different raw and cooked sausages and 80 of delicatessen and mixed salads) were examined. L. monocytogenes was identified in 52 investigated salmon samples. Using two chromogenic media, 50 samples were found positive for L. monocytogenes. Compared to the reference method there were no false-positive results. By the EN/DIN 11290-01 culture procedure after the selective enrichment in Fraser broth 12 out of 130 samples of sausages were positive for L. monocytogenes. These 12 samples were also positive for L. monocytogenes with the chromogenic medium RAPID' L. mono. One sample was false negative with ALOA. Three additional samples were found positive with ALOA and four with RAPID' L. mono. The standard method was inadequate to confirm these samples as positive. Listeria spp. were isolated from 7 sample...Continue Reading

Citations

Apr 18, 2012·International Journal of Food Microbiology·Nikolaos D AndritsosEleftherios H Drosinos
Dec 7, 2007·International Journal of Food Microbiology·Martina FrickerMonika Ehling-Schulz
May 31, 2016·Scientifica·Tünde PusztahelyiIstván Pócsi
Aug 9, 2011·Journal of Food Protection·Ulrike ZitzWolfgang Kneifel
May 3, 2014·Current Protocols in Microbiology·Reha O AzizogluSophia Kathariou

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