Comparison of two commercial quantitative PCR assays for EBV DNA detection and their correlation with the first WHO International Standard for EBV

Journal of Medical Microbiology
Ilka EngelmannDidier Hober

Abstract

There are few data on the performance of automated Epstein-Barr virus (EBV) PCR assays. This study compared EBV quantification for the kPCR PLX EBV DNA (kPCR; Siemens, France) and the EBV R-gene (R-gene; Argene, Biomerieux, France) assays and their correlation with the World Health Organization (WHO) standard. WHO International Standard for EBV (WHO standard) dilution panels in different matrices were submitted to nucleic acid extraction with Versant kPCR Molecular Systems SP followed by the kPCR assay, or to nucleic acid extraction with the MagNA Pure LC System or NucliSENS easyMag followed by the R-gene assay. Seventy-four clinical specimens were tested in both assays. Bland-Altman analysis and linear regression analysis were performed. The correlation between the WHO standard diluted in different matrices and the R-gene and kPCR assays was good (R2 >0.96 and R2 >0.92, respectively). A matrix effect was observed. The correlation of quantitative results between both assays yielded a coefficient of determination R2 higher than 0.74. The quantification differences were within one log10 of the averaged results for 34 of the 38 specimens (89 %). Calibration to the WHO standard did not increase the comparability of quantitative res...Continue Reading

References

Oct 29, 2002·Annals of Neurology·Adriana WeinbergKenneth L Tyler
Dec 17, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Donald E TsaiRichard L Hodinka
Oct 29, 2004·Nature Reviews. Cancer·Lawrence S Young, Alan B Rickinson
May 6, 2005·Journal of Clinical Microbiology·Guillermo RuizJuan Emilio Echevarría
Nov 9, 2007·Journal of Clinical Microbiology·R T HaydenUNKNOWN U.S. EBV Working Group
Jan 1, 2008·The Journal of Molecular Diagnostics : JMD·Samira Fafi-KremerJean-Marie Seigneurin
Jan 31, 2009·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J K PreiksaitisUNKNOWN American Society of Transplantation Infectious Diseases Community of Practice
Apr 17, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Barbara Gärtner, Jutta K Preiksaitis
May 28, 2010·The New England Journal of Medicine·Katherine Luzuriaga, John L Sullivan
Dec 28, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Isabella AbbateMaria R Capobianchi
Sep 20, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Milena FurioneFausto Baldanti
Mar 16, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marlinda AdhamJaap M Middeldorp
Aug 1, 2014·Journal of Clinical Microbiology·Janaki AbeynayakeBenjamin A Pinsky
Aug 14, 2015·Journal of Clinical Microbiology·R T HaydenA M Caliendo
Apr 1, 2016·Journal of Clinical Microbiology·Sara JonesShiaolan Y Ho
Apr 15, 2016·Journal of Clinical Microbiology·Touyana SemenovaRaphaële Germi
Jul 28, 2016·Biologicals : Journal of the International Association of Biological Standardization·Jacqueline F FryerUNKNOWN Collaborative Study Group
Feb 22, 2018·Diagnostic Microbiology and Infectious Disease·Ilka EngelmannDidier Hober

❮ Previous
Next ❯

Citations

Sep 8, 2018·International Journal of Cancer. Journal International Du Cancer·Victor Ho-Fun LeeAnne Wing-Mui Lee
Mar 14, 2020·Microbial Pathogenesis·Charles V OlivieriSéverine Vincent-Bugnas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.