Comparison of two different deposition methods of 3-aminopropyltriethoxysilane on glass slides and their application in the ThinPrep cytologic test

The Analyst
Sen XiangJin-Ming Lin

Abstract

In this work, two different deposition methods of 3-aminopropyltriethoxysilane (APTES) on glass slides were compared in order to study the adhesion effect of cervical exfoliated cells on smear slides. Glass slides were modified by vapor-phase deposition (V-D) and liquid-phase deposition (L-D), respectively. The topographic images and amine density of the modified slides were investigated by using atomic force microscopy, UV-vis spectroscopy and X-ray photoelectron spectroscopy. The numbers of cells adhered on the slides functionalized by V-D and L-D were counted and compared under the microscope. The data showed significant differences between the two methods (t-test: P < 0.05). The results presented here have made it theoretically possible to produce amine slides by V-D method for the ThinPrep cytologic test.

References

Oct 1, 1987·Journal of Clinical Pathology·P H Maddox, D Jenkins
Apr 1, 1987·Journal of Clinical Pathology·Y van der Graaf, G P Vooijs
May 1, 1996·Acta Cytologica·F TezukaH Higashiiwai
Sep 8, 2000·Science·T A TatonR L Letsinger
Mar 20, 2001·Journal of Biomedical Materials Research·P FilippiniM T Santini
Mar 30, 2004·Diagnostic Cytopathology·Maria da Gloria Mattosinho de Castro FerrazGeraldo Rodrigues de Lima
Jan 25, 2005·Journal of Biotechnology·Takahito NakagawaTadashi Matsunaga
Jul 13, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Ian J Bruce, Tapas Sen
Sep 28, 2006·Analytical and Bioanalytical Chemistry·Nagendra Kumar KamisettyKeisuke Makino
Dec 13, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·John A Howarter, Jeffrey P Youngblood
Jan 19, 2007·Biosensors & Bioelectronics·M Arroyo-HernándezJ M Martínez Duart
Jan 31, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Aaron S AndersonBasil I Swanson

❮ Previous
Next ❯

Citations

May 23, 2014·Angewandte Chemie·Sidharam P PujariHan Zuilhof
Aug 28, 2015·Journal of Peptide Science : an Official Publication of the European Peptide Society·Komandoor E Achyuthan, David R Wheeler
Jul 10, 2018·Advanced Materials·Yang XiaoDavid L Officer
Mar 7, 2017·Journal of Nanoparticle Research : an Interdisciplinary Forum for Nanoscale Science and Technology·Wei-Zheng ShenCarlo Montemagno
Dec 30, 2016·Future Science OA·John G HardyChristine E Schmidt
Apr 2, 2019·Colloids and Surfaces. B, Biointerfaces·Shalini R Unnikandam VeettilIan C Schneider
Aug 8, 2017·ACS Applied Materials & Interfaces·Aleksandr MarkovRoger Wördenweber

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.