Comparison of two digestion strategies on characteristics and differentiation potential of human dental pulp stem cells

Archives of Oral Biology
Maziar Ebrahimi DastgurdiMohammad Hossein Nasr-Esfahani

Abstract

This study aimed to compare the behavior of dental pulp stem cells (DPSCs) after isolation using solutions containing either collagenase/dispase or collagenase alone. DPSCs were isolated by two digestion methods (collagenase/dispase or collagenase alone) from human third molars. Immunophenotypic features were confirmed by flow cytometry for cell markers STRO-1, cluster of differentiation (CD) 146, CD45, and collagen type-I. The proliferation potential of cells was evaluated by 5-bromo-2'-deoxyuridine (brdU) incorporation assay, and finally they were assessed for multi-lineage differentiation potential. Data were analyzed using one-way analysis of variance and independent t-tests. DPSCs isolated by either method showed similar levels of STRO-1, CD45, and collagen type-I and similar incorporation of brdU (P > 0.05). However, DPSCs obtained by collagenase I/dispase treatment had significantly higher numbers of CD146+ cells and osteogenic and chondrogenic capacities compared to those obtained by treatment with collagenase I alone (P < 0.05). On the other hand, more STRO-1+/CD164-DPSCs were found in the collagenase alone group with higher adipogenic potential. Different enzyme solutions gave rise to different populations of DPSCs. D...Continue Reading

Citations

Dec 14, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Andrew Reichard, Kewal Asosingh
Dec 14, 2018·Stem Cell Research & Therapy·Xianrui YangDonghui Zhang
Nov 6, 2020·Journal of Pharmacy & Bioallied Sciences·Adlin S RosaianAnand Sherwood

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