PMID: 8940978Nov 1, 1996Paper

Comparison of two methods for enumerating malaria parasites in thick blood films

The American Journal of Tropical Medicine and Hygiene
E PetersenA Gottschau

Abstract

Calculation of parasite densities is important for estimating herd immunity to malaria, and for determining end points in field trials for interventions such as malaria vaccines, impregnated bed nets, and chemosuppression. Two methods of enumeration were compared: method 1, in which 100 consecutive high-power fields (HPFs) are examined, and if they all contain at least one parasite, the number per field is then counted in 10-100 of these fields according to density; and method 2, in which the actual number of parasites present in 100 consecutive fields are counted. The first method significantly underestimates parasite density in samples in which less than all high-power fields are parasite-positive. A correction of method 1 is suggested, which results in a parasite density, which is comparable with that obtained using method 2. The correction factor estimated was 2(-In(1 - p)), where p is the proportion of positive HPFs. The correction factor presented will allow accurate estimate of parasite densities per volume of blood even if only the proportion of parasite-positive high-power fields containing at least one parasite are counted.

Citations

Jun 19, 2012·Expert Review of Anti-infective Therapy·Patricia Schlagenhauf, Eskild Petersen
Mar 22, 2013·PloS One·Imen HammamiAndré Garcia
Jan 31, 2018·Transplantation·Lígia Camera PierrottiEskild Petersen
Feb 15, 2000·Tropical Medicine & International Health : TM & IH·A H KilianF von Sonnenburg
May 2, 2007·Annals of Tropical Paediatrics·Zaccheaus Awortu Jeremiah, Emmanuel Kufre Uko
Jan 26, 2021·Journal of Parasitology Research·Andrew Nii Adzei BekoePrecious Kwablah Kwadzokpui

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