Comparison of two mononuclear cell program settings on two apheresis devices intended to collect high yields of CD14+ and CD3+ cells

Transfusion
Erwin F StrasserReinhold Eckstein

Abstract

In cancer and transplantation therapy apheresis devices and software of optimum standards are required for the collection of high cell yields with high purity of the desired cell fraction. In a paired study, 15 healthy blood donors underwent four 10-L leukapheresis procedures (197 +/- 33 min) with an inlet blood flow rate of 60 mL per minute by use of two different MNC program settings of the COBE Spectra (Gambro BCT) and the AS.TEC 204 (Fresenius Hemocare) cell separators. Use of the standard MNC program of both apheresis devices resulted in significantly higher (p < 0.01) collection efficiencies of CD14+ monocytes, CD3+ cells, CD4+ cells, CD8+ T cells, CD16+ CD56+ natural killer (NK) cells, and residual PLTs (p < 0.001), owing to higher centrifuge speed. The mean MNC purity of all components was more than 90 percent. By use of standard programs of either device, significant correlations (p < 0.01) between donor monocytes and preleukapheresis NK cell counts and the corresponding component cell yields were found. Compared to the program modifications with lower centrifuge velocities the standard MNC programs were significantly more efficient regarding CD14+, CD3+, and CD16+ CD56+ cells. Enhanced centrifuge speed and inlet blood...Continue Reading

References

Jul 14, 2001·Transfusion·E L SnyderJ R Bender
Sep 7, 2002·Journal of Immunological Methods·Thomas G BergerBeatrice Schuler-Thurner
Nov 9, 2002·Therapeutic Apheresis : Official Journal of the International Society for Apheresis and the Japanese Society for Apheresis·A Yagihashi, K Kikuchi
Feb 6, 2003·International Journal of Hematology·Sally Arai, Hans G Klingemann
Mar 14, 2003·Current Opinion in Immunology·Gerold SchulerRalph M Steinman
Sep 3, 2003·Nature Reviews. Cancer·Mark E Dudley, Steven A Rosenberg

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Citations

Sep 15, 2007·Medicinski pregled·Ivana UrosevićStevan Popović
Jan 10, 2006·Transfusion·Erwin F StrasserReinhold Eckstein
Jul 28, 2016·Transfusion·Andreas BrosigNorbert Ahrens
Dec 2, 2018·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Adamma AnyanwuPatrick Wuchter

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