Comparison of two PCR-based human papillomavirus genotyping methods.

Journal of Clinical Microbiology
Philip E CastleCVT Group

Abstract

We compared two consensus primer PCR human papillomavirus (HPV) genotyping methods for the detection of individual HPV genotypes and carcinogenic HPV genotypes as a group, using a stratified sample of enrollment cervical specimens from sexually active women participating in the NCI/Costa Rica HPV16/18 Vaccine Efficacy Trial. For the SPF(10) method, DNA was extracted from 0.1% of the cervical specimen by using a MagNA Pure LC instrument, a 65-bp region of the HPV L1 gene was targeted for PCR amplification by using SPF(10) primers, and 25 genotypes were detected by reverse-line blot hybridization of the amplicons. For the Linear Array (LA) method, DNA was extracted from 0.5% of the cervical specimen by using an MDx robot, a 450-bp region of the HPV L1 gene was targeted for PCR amplification by using PGMY09/11 L1 primers, and 37 genotypes were detected by reverse-line blot hybridization of the amplicons. Specimens (n = 1,427) for testing by the LA method were randomly selected from strata defined on the basis of enrollment test results from the SPF(10) method, cytology, and Hybrid Capture 2. LA results were extrapolated to the trial cohort (n = 5,659). The LA and SPF(10) methods detected 21 genotypes in common; HPV16, -18, -31, -3...Continue Reading

References

Aug 19, 1999·The Journal of Pathology·J M WalboomersN Muñoz
Jan 5, 2000·Journal of Clinical Microbiology·P E GravittR J Apple
Jan 13, 2000·JAMA : the Journal of the American Medical Association·M SchiffmanA T Lorincz
May 9, 2001·The Journal of Infectious Diseases·C L PeytonC M Wheeler
Feb 7, 2003·The New England Journal of Medicine·Thomas C Wright, Mark Schiffman
Feb 7, 2003·The New England Journal of Medicine·Nubia MuñozUNKNOWN International Agency for Research on Cancer Multicenter Cervical Cancer Study Group
May 17, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Harriet RichardsonEduardo L Franco
May 19, 2005·American Journal of Clinical Pathology·Sepehr N TabriziSuzanne M Garland
Sep 7, 2006·Journal of Clinical Microbiology·Dennis van HamontWillem J G Melchers
Oct 17, 2006·The Journal of Infectious Diseases·Cosette M WheelerUNKNOWN Atypical Squamous Cells of Undetermined Significance/Low-Grade Squamous Intraepithelial Lesions Triage Study Group
Apr 3, 2007·Journal of Virological Methods·S Terence DunnMark Schiffman
Apr 4, 2007·International Journal of Cancer. Journal International Du Cancer·Jennifer S SmithGary M Clifford
May 15, 2007·The New England Journal of Medicine·UNKNOWN FUTURE II Study Group
May 15, 2007·The New England Journal of Medicine·Suzanne M GarlandUNKNOWN Females United to Unilaterally Reduce Endo/Ectocervical Disease (FUTURE) I Investigators
Aug 21, 2007·JAMA : the Journal of the American Medical Association·Allan HildesheimUNKNOWN Costa Rican HPV Vaccine Trial Group
Oct 2, 2007·American Journal of Obstetrics and Gynecology·Thomas C WrightUNKNOWN 2006 American Society for Colposcopy and Cervical Pathology-sponsored Consensus Conference
May 17, 2008·American Journal of Epidemiology·Jill KoshiolJennifer S Smith
May 17, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Patti E GravittPhilip E Castle

❮ Previous
Next ❯

Citations

Mar 26, 2009·Journal of the National Cancer Institute·Cosette M WheelerPhilip E Castle
Nov 12, 2010·Journal of Clinical Microbiology·Elizabeth A ElseChristine C Roberts
Jan 16, 2009·Journal of Clinical Microbiology·Qinghua FengLaura A Koutsky
Dec 1, 2012·Journal of Clinical Microbiology·Jill KoshiolNicolas Wentzensen
Jun 4, 2016·Iranian Red Crescent Medical Journal·Mostafa Salehi-VaziriHossein Keyvani
Dec 31, 2011·Enfermedades infecciosas y microbiología clínica·Diego DelgadoAndrés Canut
Mar 12, 2011·Diagnostic Microbiology and Infectious Disease·Rebecca ShanesmithSamuel T Dunn
Feb 5, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·D T GeraetsW G V Quint
Jul 26, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Simon R CorrieMatt Trau
Apr 20, 2011·Journal of Medical Virology·Maria Gabriella DonàAmina Vocaturo
Nov 27, 2009·Diagnostic Cytopathology·Nalini GuptaArvind Rajwanshi
Sep 11, 2012·International Journal of Cancer. Journal International Du Cancer·Anne F RositchJennifer S Smith
Dec 20, 2011·The Journal of Obstetrics and Gynaecology Research·Seung Hun SongMi Kyung Kim
Jul 26, 2014·Journal of Medical Virology·Peter GrozdanovAngel S Galabov
Nov 18, 2009·International Journal of Cancer. Journal International Du Cancer·Jill KoshiolSanford M Dawsey
Sep 3, 2011·Journal of Virological Methods·Milena CamargoManuel Alfonso Patarroyo
Aug 12, 2010·BMC Infectious Diseases·Carolina PorrasUNKNOWN Costa Rica HPV Vaccine Trial (CVT) Group
Sep 14, 2013·Sexually Transmitted Infections·Aaron C ErmelDarron R Brown
Mar 10, 2017·Expert Review of Molecular Diagnostics·Ana Gradíssimo, Robert D Burk
Aug 26, 2017·Pathology Oncology Research : POR·Eszter SzabóIstván Vályi-Nagy
Apr 14, 2012·Journal of Medical Virology·Meng-Jie YangXue-jun Ma
Aug 8, 2015·Journal of Clinical Microbiology·K CuschieriM Arbyn
Oct 20, 2010·Expert Review of Anti-infective Therapy·Mario Poljak, Boštjan J Kocjan
Aug 8, 2020·The Tohoku Journal of Experimental Medicine·Iwao KukimotoUNKNOWN MINT Study II Group
May 31, 2017·Pediatric Blood & Cancer·Kasiani C MyersMelinda Butsch Kovacic
Aug 25, 2010·International Journal of Cancer. Journal International Du Cancer·Koji MatsumotoUNKNOWN Japan HPV And Cervical Cancer Study Group
Mar 27, 2015·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Sharon L SauterMelinda Butsch Kovacic
Apr 4, 2021·Cancers·Sharon L SauterMelinda Butsch Kovacic

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.