PMID: 2110947May 8, 1990Paper

Comparison of two serum bactericidal assays for Neisseria gonorrhoeae

Journal of Immunological Methods
R K PettitR C Judd

Abstract

Pooled normal human serum killing of 14 strains of Neisseria gonorrhoeae was assessed by dilution plate and microtiter methods. In both assays, the strains presented a spectrum of sensitivity to the serum. In the dilution plate assay, results with two different concentrations of human serum were similar for most, but not all of the strains tested. When data for all of the strains were compared, no correlation was found between the dilution plate and microtiter bactericidal assays. Finally, we found that the bactericidal capacities of intact and complement-depleted human sera were very similar when assessed by microtiter methods, suggesting a non-complement-mediated serum killing mechanism.

References

Mar 1, 1986·The Journal of Infectious Diseases·M A ApicellaJ M Griffiss
Jan 1, 1982·Journal of General Microbiology·H SchneiderG B Pier
Oct 15, 1982·Journal of Immunological Methods·H Schneider, J M Griffiss
Oct 13, 1981·Biochemistry·S F Carroll, R J Martinez

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Citations

Jan 1, 1991·Journal of Clinical Microbiology·R J ArkoS A Morse
Mar 1, 1996·Infection and Immunity·R K PettitE S Martin
Jul 1, 1995·Infection and Immunity·R K PettitV J Bertolino

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