Comparison of two substrate materials used as negative control in endothelialization studies: Glass versus polymeric tissue culture plate

Clinical Hemorheology and Microcirculation
Anne Krüger-GengeFriedrich Jung

Abstract

The endothelialization of synthetic surfaces applied as cardiovascular implant materials is an important issue to ensure the anti-thrombotic quality of a biomaterial. However, the rapid and constant development of a functionally-confluent endothelial cell monolayer is challenging. In order to investigate the compatibility of potential implant materials with endothelial cells several in vitro studies are performed. Here, glass and tissue culture plates (TCP) are often used as reference materials for in vitro pre-testing. However, a direct comparison of both substrates is lacking.Therefore, a comparison of study results is difficult, since results are often related to various reference materials. In this study, the endothelialization of glass and TCP was investigated in terms of adherence, morphology, integrity, viability and function using human umbilical vein endothelial cells (HUVEC).On both substrates an almost functionally confluent HUVEC monolayer was developed after nine days of cell seeding with clearly visible cell rims, decreased stress fiber formation and a pronounced marginal filament band. The viability of HUVEC was comparable for both substrates nine days after cell seeding with only a few dead cells. According to t...Continue Reading

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Citations

Sep 11, 2019·International Journal of Molecular Sciences·Anne Krüger-GengeFriedrich Jung
Feb 6, 2021·International Journal of Molecular Sciences·Christian SchulzFriedrich Jung
Dec 3, 2020·International Journal of Molecular Sciences·Steffen BrauneFriedrich Jung

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