Comparison of type III secretion system virulence among fluoroquinolone-susceptible and -resistant clinical isolates of Pseudomonas aeruginosa

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
Annie Wong-BeringerJ Flanagan

Abstract

Fluoroquinolone resistance and type III secretion system (TTSS) virulence are independently associated in Pseudomonas aeruginosa infections with poor patient outcomes. In the present study, the virulence of fluoroquinolone-susceptible and -resistant isolates of P. aeruginosa was compared, focusing on TTSS virulence. Clinical isolates (n = 45) exhibiting a broad range of susceptibilities to fluoroquinolones, with differing mechanisms of resistance and associated with varying disease sites, were selected for the study. PCR, Southern blot and western immunoblot analyses were performed to determine the presence of TTSS-encoding genes and secretion of gene products. The cytotoxicity of the clinical isolates towards human lung epithelial cells was also determined. Clinical isolates encoding only the exoS cytotoxin gene occurred more frequently than those encoding only exoU (62% vs. 27%; p 0.0007). Compared with exoS(+) isolates, exoU(+) isolates were more likely to be fluoroquinolone-resistant (92% vs. 61%, p 0.05) and to exhibit both a gyrA mutation and the efflux pump over-expressed (EPO) phenotype (91% vs. 59%; p 0.06). Almost all exoU(+) strains secreted ExoU and exhibited increased cytotoxicity compared with ExoS-secreting strai...Continue Reading

References

Jun 1, 1993·Trends in Microbiology·C J Dorman, N Ní Bhriain
Aug 22, 2000·Antimicrobial Agents and Chemotherapy·K Poole
May 24, 2001·The Journal of Infectious Diseases·A Roy-BurmanJ P Wiener-Kronish
Jul 12, 2001·Proceedings of the National Academy of Sciences of the United States of America·J R FitzgeraldJ M Musser
Feb 18, 2003·JAMA : the Journal of the American Medical Association·Melinda M NeuhauserJohn P Quinn
Jun 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Matthew C WolfgangStephen Lory
Sep 25, 2004·Molecular Microbiology·Hiromi Sato, Dara W Frank
Nov 24, 2004·Infection and Immunity·Ciara M Shaver, Alan R Hauser
Mar 5, 2005·The American Journal of Medicine·Jeffrey A LinderRandall S Stafford
Jun 9, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·George A Jacoby

❮ Previous
Next ❯

Citations

Mar 28, 2009·Critical Care Medicine·Jeffrey L VeesenmeyerJordi Rello
Nov 15, 2011·Critical Care Medicine·Ali A El-SolhHardik Vora
Aug 21, 2008·American Journal of Respiratory and Critical Care Medicine·Alan R Hauser
Aug 21, 2012·PloS One·Melissa Agnello, Annie Wong-Beringer
Feb 13, 2016·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Masaru ShimizuTeiji Sawa
Dec 24, 2015·Microbiology and Immunology·Hiroaki YasumotoTeiji Sawa
Sep 22, 2012·Burns : Journal of the International Society for Burn Injuries·Fereshteh JabalameliMohammad Emaneini
Feb 13, 2015·Critical Care : the Official Journal of the Critical Care Forum·Teiji SawaJeanine P Wiener-Kronish
May 1, 2010·Journal of Medical Microbiology·R S BradburyA C Champion
Dec 9, 2014·Human Vaccines & Immunotherapeutics·Teiji SawaMatthew Haight
Nov 8, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Carmen PeñaUNKNOWN Spanish Network for Research in Infectious Diseases (REIPI)
Apr 5, 2013·Clinical Microbiology Reviews·Alejandro BeceiroGermán Bou
Sep 13, 2017·Journal of Medical Microbiology· AditiMujeeb-Ur Rahman
Nov 29, 2013·Avian Diseases·J Michael Day, Laszlo Zsak
Dec 8, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·K Adam MorrowTroy Stevens
Jan 23, 2020·Microbiology and Immunology·Teiji SawaKiyoshi Moriyama
Apr 5, 2017·The Journal of Infectious Diseases·Carlos JuanAntonio Oliver
Jul 16, 2019·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Y-H Chai, J-F Xu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.