Comparison of typing methods with a new procedure based on sequence characterization for Salmonella serovar prediction

Journal of Clinical Microbiology
Matthew Louis RanieriMartin Wiedmann


As the development of molecular serotyping approaches is critical for Salmonella spp., which include >2,600 serovars, we performed an initial evaluation of the ability to identify Salmonella serovars using (i) different molecular subtyping methods and (ii) a newly implemented combined PCR- and sequencing-based approach that directly targets O- and H-antigen-encoding genes. Initial testing was performed using 46 isolates that represent the top 40 Salmonella serovars isolated from human and nonhuman sources, as reported by the U.S. Centers for Disease Control and Prevention and the World Health Organization. Multilocus sequence typing (MLST) was able to accurately predict the serovars for 42/46 isolates and showed the best ability to predict serovars among the subtyping methods tested. Pulsed-field gel electrophoresis (PFGE), ribotyping, and repetitive extragenic palindromic sequence-based PCR (rep-PCR) were able to accurately predict the serovars for 35/46, 34/46, and 30/46 isolates, respectively. Among the methods, S. enterica subsp. enterica serovars 4,5,12:i:-, Typhimurium, and Typhimurium var. 5- were frequently not classified correctly, which is consistent with their close phylogenetic relationship. To develop a PCR- and se...Continue Reading


Feb 28, 2014·Trends in Microbiology·Teresa M BergholzMartin Wiedmann
Jul 26, 2014·Emerging Infectious Diseases·Henk C den BakkerWilliam J Wolfgang
Jul 25, 2015·International Journal of Food Microbiology·Chien-Shun ChiouYen-Yi Liu
Apr 14, 2016·PeerJ·Philip M AshtonSalmonella Whole Genome Sequencing Implementation Group
Jan 18, 2015·Journal of Microbiological Methods·Joelle K SalazarWei Zhang
Nov 16, 2013·Critical Reviews in Microbiology·Chunlei ShiAndrea I Moreno-Switt
Oct 18, 2016·Frontiers in Microbiology·Wenyao ChenChunlei Shi
Jan 20, 2018·Annual Review of Food Science and Technology·Francesca De FilippisDanilo Ercolini
Jan 10, 2018·Frontiers in Microbiology·Rafaela G FerrariCarlos A Conte-Junior
Apr 22, 2014·Applied and Environmental Microbiology·Laura K StrawnMartin Wiedmann
Jun 28, 2015·Applied and Environmental Microbiology·Matthew J StasiewiczHenk C den Bakker
Apr 4, 2020·Journal of Wildlife Diseases·Rodolfo TardoneAndrea I Moreno-Switt
Oct 2, 2020·Molecular Biology Reports·Jobin John JacobBalaji Veeraraghavan
Nov 27, 2015·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Sonia KtariAdnene Hammami
Mar 28, 2019·Journal of Food Protection·National Advisory Committee On Microbiological Criteria For Foods
Aug 27, 2018·EFSA Journal·UCD Centre for Food Safety, Dublin, IrelandF Butler
Jul 30, 2019·Frontiers in Microbiology·Silin TangMartin Wiedmann


Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M SilvermanM Simon
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Sep 1, 1985·Annales De L'Institut Pasteur. Microbiology·M Y Popoff, L Le Minor
Feb 1, 1993·Journal of Clinical Microbiology·E EstebanH Kinde
Dec 1, 1996·Trends in Microbiology·P R ReevesP D Rick
Mar 7, 2002·Journal of Clinical Microbiology·Geeta Mehta, Subhash C Arya
May 1, 2002·Journal of Clinical Microbiology·Mamuka KotetishviliAlexander Sulakvelidze
Jul 3, 2002·Journal of Food Protection·J S BaileyD E Cosby
Jun 12, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Claire KidgellMark Achtman
May 8, 2004·Journal of Clinical Microbiology·John R McQuistonPatricia I Fields
Aug 10, 2004·BMC Microbiology·Chloe K B MortimerCatherine Arnold
Feb 5, 2005·BMC Infectious Diseases·Dirk WerberAndrea Ammon
May 18, 2006·Emerging Infectious Diseases·Eleni GalanisWorld Health Organization Global Salm-Surv
Dec 15, 2006·Journal of Clinical Microbiology·Stephen B GaulLorraine Hoffman
Jan 30, 2007·Research in Microbiology·Silvia Herrera-LeónMaria Aurora Echeita
Jun 9, 2007·Journal of Microbiological Methods·Catherine YoshidaKris Rahn
Jul 20, 2007·Journal of Clinical Microbiology·Collette FitzgeraldPatricia I Fields
Sep 22, 2007·Foodborne Pathogens and Disease·Annaëlle KérouantonAnne Brisabois
Mar 29, 2008·Briefings in Bioinformatics·Kazutaka Katoh, Hiroyuki Toh
Jun 6, 2008·Journal of Clinical Microbiology·Nabil ArrachMichael McClelland
Oct 7, 2008·Journal of Microbiological Methods·Mark G WiseBruce S Seal
Feb 25, 2009·International Journal of Food Microbiology·N C ElvissFood, Water and Environmental Surveillance Network
Mar 19, 2009·Foodborne Pathogens and Disease·Andrea I Moreno SwittMartin Wiedmann
Oct 21, 2009·Research in Microbiology·Martine GuibourdencheFrançois-Xavier Weill
Feb 18, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shannon E MajowiczInternational Collaboration on Enteric Disease 'Burden of Illness' Studies
Feb 26, 2010·Foodborne Pathogens and Disease·Yeşim SoyerMartin Wiedmann
Jul 16, 2010·Journal of Clinical Microbiology·Wen ZouJames J Chen
Oct 19, 2010·Epidemiology and Infection·N NicolayD O'Flanagan
Dec 17, 2010·Journal of Clinical Microbiology·John R McQuistonPatricia I Fields
Jun 24, 2011·Journal of Clinical Microbiology·Kristyn FranklinMohamed A Karmali

Related Concepts

H antigen, bacterial
Salmonella enterica
Antigens, Bacterial
Sequence Determinations, DNA
Salmonella Infections

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.