Comparison of Ultrasound-Assisted Headspace Solid-Phase Microextraction and Hydrodistillation for the Identification of Major Constituents in Two Species of Hypericum

Journal of Chromatographic Science
Alireza GhiasvandPayman Hashemi

Abstract

Ultrasound-assisted headspace solid-phase microextraction (UA-HS-SPME) and hydrodistillation (HD) methods, coupled to gas chromatography-mass spectrometry (GC-MS), were used for the extraction and analysis of volatile compounds from Hypericum perforatum and Hypericum scabrum collected from two different sites in Iran. In the UA-HS-SPME method, various experimental parameters such as the type of fiber coating, sonication time, extraction time, extraction temperature and desorption time were investigated. The highest extraction efficiency was achieved by a 100-µm polydimethylsiloxane (PDMS) fiber. Consequently, 36 compounds were identified in H. perforatum and H. scabrum samples, using the UA-HS-SPME-GC-MS method, of which 14 were the same in both species. On the other hand, 57 compounds of these species were identified by the HD-GC-MS method, of which 21 were the same. The predominant constituents identified using the UA-HS-SPME method in H. perforatum included β-caryophyllene, α-pinene, γ-cadinene, α-selinene, germacrene-D, limonene and myrcene, and in H. scabrum were α-pinene, nonane, β-pinene and limonene. The common constituents identified by the HD-GC-MS method for H. perforatum involved germacrene-D, limonene, β-caryophyll...Continue Reading

References

Jul 13, 2000·Journal of Chromatography. a·H KataokaJ Pawliszyn
Aug 21, 2003·Journal of Agricultural and Food Chemistry·Seied Mahdi PourmortazaviSeied Ghorban Hosseini
Dec 6, 2005·Phytochemistry·Andrija SmelcerovicGhulam Nabi Qazi
Jul 13, 2007·Journal of Chromatography. a·Seied Mahdi Pourmortazavi, Seiedeh Somayyeh Hajimirsadeghi
Apr 30, 2007·Talanta·M D Luque de Castro, F Priego-Capote
Mar 21, 2009·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Axel Brattström
Nov 13, 2009·Pharmaceutical Biology·Diane F BirtJason P Price
Mar 26, 2013·Journal of the Science of Food and Agriculture·Lavinia AlexandruGiancarlo Cravotto

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Citations

May 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sana AbdolhosseiniNahid Heidari

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