PMID: 6412622Apr 1, 1983Paper

Comparison of various atmospheric conditions for isolation and subcultivation of Mycoplasma hyorhinis from cell cultures

Antonie van Leeuwenhoek
A A Polak-VogelzangJ Borst

Abstract

The efficiency of aerobic incubation was compared with incubation under various oxygen and carbon dioxide conditions for the isolation and subcultivation of three strains of Mycoplasma hyorhinis from VERO-cell cultures and subcultivation of three laboratory strains. Under anaerobic conditions with a low oxidation-reduction potential (at or below -115 mV) as obtained in jars, with catalysts, containing mixtures of 5%-10% CO2 in H2, very poor or no growth of any of the six M. hyorhinis strains was observed. When traces of oxygen were present (that is, under conditions with higher oxidation-reduction potentials, e.g. when omitting the catalyst in the above gas mixtures or in 5% CO2 + 95% N2) isolation from cell cultures was successful in most tests, but subcultivation of these primary isolates was seldom possible under these semi-anaerobic conditions. However, in most cases these primary isolates could be subcultivated aerobically, although aerobic conditions were unsatisfactory for isolation in about half of the experiments. Isolation of M. hyorhinis was optimal in 5% O2 + 95% N2, under which condition the isolates could also always be subcultivated. Isolation failed occasionally when 5% O2 + 5% CO2 + 90% N2 was used, thus indica...Continue Reading

References

Jul 1, 1973·In Vitro·G J McGarrity, L L Coriell
Jul 28, 1967·Annals of the New York Academy of Sciences·J F Hers, N Masurel
Mar 1, 1980·Journal of Clinical Microbiology·W F Seip, G L Evans
Jan 1, 1980·Journal of Biological Standardization·A A Polak-VogelzangF E Hekkens
Jan 1, 1963·Antonie van Leeuwenhoek·D HERDERSCHEE
Feb 1, 1964·Journal of General Microbiology·M BUTLER, R H LEACH
Jan 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·J F MORGANR C PARKER

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Citations

Jan 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H KotaniG J McGarrity
Apr 1, 1990·Biologicals : Journal of the International Association of Biological Standardization·A A Polak-VogelzangR Reijgers
Jan 15, 2011·Molecular and Cellular Probes·Dmitriy V VolokhovVladimir E Chizhikov
May 1, 1989·Research in Virology·C Bonissol, B Stoiljkovic
Sep 1, 1996·Applied and Environmental Microbiology·M KonaiR F Whitcomb

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