Abstract
Growth factor based angiogenesis, with or without cell therapy, is a promising therapeutic modality for patients with coronary artery disease. We compared the relative efficacies of surgically delivered vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2) in a swine model of hypercholesterolemia-induced endothelial dysfunction which captures many of the pathophysiologic abnormalities of human coronary disease. Yucatan mini-swine (20-30 kg), fed a high cholesterol diet (total 20 weeks), underwent circumflex ameroid placement to create chronic myocardial ischemia, followed three weeks later by perivascular administration of VEGF (2 microg; n=6), FGF-2 (100 microg; n=6), or placebo (n=7) in the ischemic territory. Normocholesterolemic animals (n=7) served as controls. Four weeks later, endothelial function, collateral-dependent perfusion, as well as myocardial protein and mRNA levels of angiogenic mediators were assessed. Endothelial dysfunction was observed in all hypercholesterolemic animals as impaired microvessel relaxation in response to adenosine diphosphate and VEGF. VEGF administration improved baseline-adjusted collateral-dependent perfusion at rest (-0.03+/-0.05 vs -0.12+/-0.04, VEGF vs placeb...Continue Reading
References
Jan 24, 1997·Cell·M S O'ReillyJ Folkman
Jul 1, 1998·The Annals of Thoracic Surgery·F W SellkeM Simons
Dec 2, 1998·The Journal of Thoracic and Cardiovascular Surgery·M TofukujiF W Sellke
Mar 8, 2000·Atherosclerosis·X ZhangQ C Zhou
Sep 12, 2000·Circulation·M SimonsT K Rosengart
Sep 20, 2000·Circulation·J J JangJ P Cooke
Nov 14, 2000·Circulation Research·H Cai, D G Harrison
Dec 31, 2002·Cardiovascular Research·Karen A DetillieuxPeter A Cattini
Jan 25, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Yufeng ZhangMichael Simons
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Sep 13, 2003·Circulation·Marc RuelFrank W Sellke
Mar 3, 2004·The Annals of Thoracic Surgery·G Chad HughesKevin P Landolfo
Aug 10, 2004·Surgery·Pierre VoisineFrank W Sellke
Sep 15, 2005·Circulation·Pierre VoisineFrank W Sellke
Jan 24, 2006·The Annals of Thoracic Surgery·Munir BoodhwaniFrank W Sellke
May 27, 2006·Biochemical and Biophysical Research Communications·Melissa CudmoreAsif Ahmed
Jul 6, 2006·Circulation·Munir BoodhwaniFrank W Sellke
Sep 19, 2006·Shock·Munir BoodhwaniFrank W Sellke
Dec 5, 2006·The Journal of Thoracic and Cardiovascular Surgery·Munir BoodhwaniFrank W Sellke
Citations
Aug 31, 2012·World Journal of Urology·Waldemar S CostaFrancisco J B Sampaio
Jun 30, 2011·Basic Research in Cardiology·Antonio D LassalettaFrank W Sellke
Feb 14, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gabor M Rubanyi
Feb 4, 2009·Antioxidants & Redox Signaling·Munir Boodhwani, Frank W Sellke
Jan 15, 2011·Journal of Cardiovascular Pharmacology·Michael P RobichFrank W Sellke
Nov 1, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Louis M ChuFrank W Sellke
Mar 22, 2012·Journal of Vascular Research·Angela F Messmer-BlustJian Li
Sep 21, 2010·Circulation·Michael P RobichFrank W Sellke
Jan 19, 2011·Reproductive Biology and Endocrinology : RB&E·Andrew K EdwardsChandrakant Tayade
Aug 8, 2012·Journal of the American College of Surgeons·Michael P RobichFrank W Sellke
Sep 1, 2011·Surgery·Louis M ChuFrank W Sellke
Sep 10, 2010·Journal of Molecular and Cellular Cardiology·Michael P RobichFrank W Sellke
Jun 24, 2010·Surgery·Michael P RobichFrank W Sellke
Aug 24, 2010·Journal of the American College of Surgeons·Michael P RobichFrank W Sellke
Dec 25, 2013·Circulation Journal : Official Journal of the Japanese Circulation Society·Antonio D LassalettaFrank W Sellke
Sep 18, 2012·Journal of Molecular and Cellular Cardiology·Robina MatyalFrank W Sellke
Dec 6, 2017·Molecular Medicine Reports·Lin LingLiucheng Ding
Mar 10, 2017·Expert Opinion on Biological Therapy·Kay MaedaMarc Ruel
Jul 12, 2020·Stem Cell Research & Therapy·Yi HuangZhongqing Wei
Nov 9, 2011·Expert Review of Cardiovascular Therapy·Michael P RobichFrank W Sellke