Comparison of Vero cell assay and PCR as indicators of the presence of verocytotoxigenic Escherichia coli in bovine and human fecal samples.

Applied and Environmental Microbiology
K RahnR P Johnson

Abstract

Comparisons were made between Vero cell assay (VCA) and PCR as indicators for the detection of verocytotoxigenic Escherichia coli (VTEC; also known as Shiga-like toxin-producing E. coli) and as predictors of VTEC isolation from bovine and human fecal samples. Fecal samples were collected as part of a survey on the prevalence of VTEC on dairy farms in southern Ontario (J. B. Wilson et al., J. Infect. Dis., 174:1021-1027, 1996). A total of 2,655 samples were examined by VCA and PCR, 2,153 originating from cattle and 502 originating from humans. Overall, 36.2% of the samples were positive in the VCA and 38.7% were positive by PCR. Of the VCA-positive samples screened, 41.6% yielded a VTEC isolate. For both human and bovine samples, a significant positive association between PCR result and VCA titer (P = 0.0001) was found. In addition, there was a significant positive association between the PCR result and VTEC isolation from VCA-positive samples for cattle (odds ratio = 9.1, P < 0.0001). For bovine samples positive in the VCA, VCA titer was significantly associated with the probability of obtaining a VTEC isolate. Agreement between VCA and PCR was good for both bovine and human samples (kappa = 0.69 and 0.64, respectively). The se...Continue Reading

References

Dec 1, 1977·Infection and Immunity·J KonowalchukS Stavric
Sep 1, 1992·International Journal of Food Microbiology·L RossenO F Rasmussen
Apr 1, 1990·Applied and Environmental Microbiology·O SuthienkulP Echeverria
Nov 1, 1990·The Journal of Infectious Diseases·D R PollardK R Rozee
Dec 1, 1982·The Journal of Infectious Diseases·A D O'BrienS B Formal
Oct 1, 1993·Applied and Environmental Microbiology·K L JosephsonI L Pepper
Dec 1, 1993·Applied and Environmental Microbiology·D G Milley, L H Sekla
Nov 1, 1996·The Journal of Infectious Diseases·J B WilsonJ S Spika

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Citations

Apr 7, 2005·Applied and Environmental Microbiology·Yadilka MaldonadoArun K Bhunia
Oct 3, 2009·Journal of Applied Microbiology·E P BlackK E Kniel
Aug 18, 2009·Applied and Environmental Microbiology·István TóthBéla Nagy
Apr 20, 2021·International Journal of Microbiology·Indira T KudvaClint A Loest
Feb 8, 2005·Journal of Clinical Microbiology·P L MelitoL K Ng
Oct 6, 2005·Applied and Environmental Microbiology·Tina TreudeAntje Boetius

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