Comparison of vesicular volume and quantal size in bovine chromaffin cells

Neuroscience
M I GlavinovićJ M Trifaró

Abstract

Electrochemical measurements of vesicular content released were compared with the morphometric measurements of vesicular size in bovine chromaffin cells. Cross-sectional vesicular diameters were determined from electron micrographs. Two methods were used to determine the frequency histograms of "true" vesicular diameters (i.e. diameters of the vesicles in the equatorial plane): (i) "peeling off" method [Coupland R. E. (1968), Nature 217, 384-388], and (ii) summation of individual probabilities of "true" vesicular diameters. Quantal size was estimated from the area under the spontaneous current spike detected electrochemically. The frequency histograms of "true" vesicular diameters are found to be skewed (thus not well described by a Gaussian function) irrespective of the method used to calculate them, as are the frequency histograms of the cube roots of the quantal sizes. Furthermore, we also find that the frequency histograms of electrochemical measurements (the cube roots of quantal sizes) have lower skews and coefficients of variation than those of morphometric measurements ("true" vesicular diameters), with discrepancy being especially pronounced for noradrenaline-secreting cells. Such a difference in both coefficients of v...Continue Reading

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Citations

Jul 12, 2002·Journal of Neuroscience Research·Nicole C BerchtoldCarl W Cotman
Mar 22, 2006·Biological cybernetics·H R RabieM I Glavinović
Mar 16, 2000·Neuroscience·L M KovalE A Lukyanetz
Aug 25, 2005·Nature Methods·Eugene V Mosharov, David Sulzer
Sep 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shiv K SharmaThomas J Carew
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·D D MurphyM Segal
Oct 31, 2002·Reviews in the Neurosciences·Réne Garcia
Jan 22, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Diana B Peckys, Niels de Jonge
Feb 7, 2001·Biochemical and Biophysical Research Communications·R H WesterinkH P Vijverberg
Feb 20, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Diana B Peckys, Niels de Jonge
Apr 26, 2011·Journal of Structural Biology·Yasumi OhtaYoshinori Fujiyoshi
Nov 18, 2005·Journal of Neuroscience Methods·D GermainM I Glavinovic
Mar 16, 2000·Reviews in the Neurosciences·D Sulzer, E N Pothos
Nov 4, 2009·Biophysical Journal·Kristi L BudzinskiDaniel T Chiu
Oct 15, 2018·FEBS Letters·Erdem Karatekin
Jun 10, 2005·Journal of Neurophysiology·Chad P GrabnerAaron P Fox
Mar 16, 2001·Analytical Chemistry·C F WilsonR N Zare

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