PMID: 2984325Mar 1, 1985Paper

Comparison of Western Blot Analysis to microneutralization for the detection of type-specific herpes simplex virus antibodies

Journal of Medical Virology
D I BernsteinY J Bryson

Abstract

A newly developed system (as described in the previous paper) employing separated herpes simplex virus (HSV) polypeptides electrophoretically transferred to nitrocellulose paper, Western Blot Analysis (WBA), was compared to microneutralization (MN) for the detection of type-specific antibody to HSV types 1 and 2 in 98 human sera. Sera containing HSV1-specific antibodies reacted at least twofold more densely (as quantitated by densitometric scanning) to HSV1 than HSV2 polypeptides, while sera with HSV2-specific antibodies reacted twofold more densely to HSV2 polypeptides. The reliability of this system was determined (1) by analysis of sera from patients with true primary herpes simplex infections, (2) by adsorption studies that removed cross-reacting antibodies, and (3) by comparison with neutralization. Of 36 sera found to have HSV1-type-specific antibody by WBA, 83% had MN ratios of less than 85 (indicating HSV1 antibody), while of 28 sera found to have HSV2-type-specific antibody by WBA, 89% had MN ratios of greater than 85 (indicating HSV2 antibodies). The MN ratio obtained on sera felt to have both HSV1-type-specific and HSV2-type-specific antibody by WBA ranged from 67 to 160. WBA agreed more closely with clinical isolate...Continue Reading

Citations

Jan 1, 1990·Journal of Medical Virology·D I BernsteinM G Myers
Nov 1, 1987·Journal of Virological Methods·R L Ashley, J Militoni
Oct 1, 1990·Journal of Virological Methods·D R HarperH O Kangro
Nov 17, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Lawrence R StanberryDavid I Bernstein
Oct 27, 2004·Sexually Transmitted Diseases·Kenneth H FifeLawrence R Stanberry
Oct 1, 1987·American Journal of Public Health·T L KuhlsJ D Cherry

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