PMID: 8938518Oct 1, 1996Paper

Comparison of whole serum-deprived media for ex vivo expansion of hematopoietic progenitor cells from cord blood and mobilized peripheral blood mononuclear cells

Journal of Hematotherapy
C E SandstromW M Miller

Abstract

A whole serum-deprived (WSD) medium was developed and optimized for expansion of colony-forming cells (CFC) in cord blood (CB) mononuclear cell (MNC) cultures. This medium was compared with four commercially available WSD media (commercial media), three WSD media whose compositions have been publicly disclosed (public media), two serum-containing media, and two basal media, for cell and CFC expansion in 10-day CB and mobilized peripheral blood (PB) MNC cultures supplemented with interleukin-3 (IL-3), IL-6, and stem cell factor (SCF). Selected WSD media and both serum-containing media gave significant CFC expansion in CBMNC and PBMNC cultures. The serum-containing human long-term medium gave the greatest cell (3.0-fold) and CFC (25-fold) expansions in CBMNC cultures, whereas our medium maintained the most cells (93% of input) and gave the greatest CFC expansion (6.1-fold) for PBMNC cultures. Of the commercial media, Progenitor-34 gave the greatest cell expansion (1.2-fold) and X VIVO-10 gave the greatest CFC expansion (11-fold) for CBMNC cultures, and Progenitor-34 maintained the most cells (83% of input) and gave the greatest CFC expansion (3.1-fold) for PBMNC cultures. Of the public media (including ours), our medium gave the ...Continue Reading

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Citations

Sep 30, 2000·Stem Cells·L KanzS Scheding
Jun 23, 2005·Biotechnology and Bioengineering·Yan LiRamkumar Mandalam
Jun 4, 2002·Cytotherapy·C A Keever-Taylor
Apr 27, 2012·PloS One·Cristina MazzaccaraLucia Sacchetti
Nov 10, 2001·Annual Review of Biomedical Engineering·L K Nielsen
Jun 9, 2009·Trends in Biotechnology·Nicholas E Timmins, Lars K Nielsen
Sep 10, 2016·PloS One·Savit B PrabhuShinjini Bhatnagar
Jul 18, 2016·European Journal of Medicinal Chemistry·Francisco Cortés-BenítezEugene Bratoeff
Aug 28, 2012·Journal of Immunological Methods·Amanda A P NgJohn E Connolly
Apr 1, 1999·Biotechnology and Bioengineering·D MöbestR Henschler
Oct 16, 2004·Biotechnology and Bioengineering·Muhammad A S ChaudhryJames M Piret

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