Abstract
A whole serum-deprived (WSD) medium was developed and optimized for expansion of colony-forming cells (CFC) in cord blood (CB) mononuclear cell (MNC) cultures. This medium was compared with four commercially available WSD media (commercial media), three WSD media whose compositions have been publicly disclosed (public media), two serum-containing media, and two basal media, for cell and CFC expansion in 10-day CB and mobilized peripheral blood (PB) MNC cultures supplemented with interleukin-3 (IL-3), IL-6, and stem cell factor (SCF). Selected WSD media and both serum-containing media gave significant CFC expansion in CBMNC and PBMNC cultures. The serum-containing human long-term medium gave the greatest cell (3.0-fold) and CFC (25-fold) expansions in CBMNC cultures, whereas our medium maintained the most cells (93% of input) and gave the greatest CFC expansion (6.1-fold) for PBMNC cultures. Of the commercial media, Progenitor-34 gave the greatest cell expansion (1.2-fold) and X VIVO-10 gave the greatest CFC expansion (11-fold) for CBMNC cultures, and Progenitor-34 maintained the most cells (83% of input) and gave the greatest CFC expansion (3.1-fold) for PBMNC cultures. Of the public media (including ours), our medium gave the ...Continue Reading
References
Oct 26, 1978·Nature·J S Greenberger
Jun 1, 1992·The Journal of Experimental Medicine·P M Lansdorp, W Dragowska
Sep 1, 1991·British Journal of Haematology·L DouayN C Gorin
Oct 1, 1989·British Journal of Haematology·X DrouetA Najman
Dec 1, 1988·Experientia·H BakerO Frank
Dec 22, 1988·The New England Journal of Medicine·S A RosenbergC A Seipp
Apr 9, 1987·The New England Journal of Medicine·S A RosenbergJ T Rubin
Oct 1, 1987·British Journal of Haematology·G Migliaccio, A R Migliaccio
Dec 24, 1987·Journal of Immunological Methods·L M MuulS A Rosenberg
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J F Eliason, N Odartchenko
Nov 1, 1971·In Vitro·C W BooneR E Stevenson
Dec 1, 1974·British Journal of Haematology·T M Dexter, L G Lajtha
Nov 1, 1984·International Journal of Cell Cloning·S Merchav, G Wagemaker
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S Gartner, H S Kaplan
Mar 1, 1980·Experimental Cell Research·N N IscoveC Weyman
Feb 11, 1993·Nucleic Acids Research·V A MalkovM D Frank-Kamenetskii
Apr 5, 1994·Biotechnology and Bioengineering·C E SandstromE T Papoutsakis
Citations
Oct 13, 2001·Cancer Letters·G AgathaF Zintl
Feb 13, 2001·British Journal of Haematology·L LazzariG Sirchia
Mar 17, 2001·Birth·F O Smith, B G Thomson
Mar 29, 2014·Toxicology and Applied Pharmacology·Prarthana ChakrabortySyed N Kabir
May 26, 1999·Stem Cells·D MöbestR Henschler
Jan 1, 1997·Stem Cells·W BruggerL Kanz
Jan 1, 1997·Stem Cells·J A LaIuppaW M Miller
Sep 30, 2000·Stem Cells·L KanzS Scheding
Jun 23, 2005·Biotechnology and Bioengineering·Yan LiRamkumar Mandalam
Jun 4, 2002·Cytotherapy·C A Keever-Taylor
Jul 26, 2003·Cytotherapy·M GuoJ N Winter
Apr 27, 2012·PloS One·Cristina MazzaccaraLucia Sacchetti
Nov 10, 2001·Annual Review of Biomedical Engineering·L K Nielsen
Jun 9, 2009·Trends in Biotechnology·Nicholas E Timmins, Lars K Nielsen
May 8, 2010·Veterinary Immunology and Immunopathology·Chris P VerschoorNiel A Karrow
Sep 10, 2016·PloS One·Savit B PrabhuShinjini Bhatnagar
Jul 18, 2016·European Journal of Medicinal Chemistry·Francisco Cortés-BenítezEugene Bratoeff
Aug 28, 2012·Journal of Immunological Methods·Amanda A P NgJohn E Connolly
Apr 1, 1999·Biotechnology and Bioengineering·D MöbestR Henschler
Jan 5, 2002·Transfusion·A C LamP M Yuen
Oct 16, 2004·Biotechnology and Bioengineering·Muhammad A S ChaudhryJames M Piret
Apr 1, 1999·Biotechnology and Bioengineering·P C CollinsE T Papoutsakis
Apr 1, 1999·Biotechnology and Bioengineering·C L McDowell, E T Papoutsakis
Nov 26, 1998·Journal of Hematotherapy·M R KollerA K Smith
Jul 5, 2001·The Journal of Gene Medicine·J S DandoC Bordignon
Jul 14, 2001·Vox Sanguinis·K LiP M Yuen