Comparison of Whole-Tissue and Xylem Fluid Collection Techniques to Detect Xylella fastidiosa in Grapevine and Oleander

Plant Disease
Blake Bextine, Thomas A Miller

Abstract

Xylella fastidiosa is the xylem-limited bacterium that causes Pierce's disease of grapevine and oleander leaf scorch. Detection of this pathogen prior to symptom development is critical for improved management of the disease. Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) currently are used for routine detection of the pathogen; however, both detection methods are limited by low titer or patchy distribution of the bacterium within a host plant. In the study reported here, we directly compared X. fastidiosa detection in whole-tissue samples with xylem fluid samples from grapevine and oleander. Collection of xylem fluid samples improved sensitivity of pathogen detection by ELISA (41.0%) compared with whole-tissue samples (20.5%) in asymptomatic grapevine. Additionally, pathogen detection in asymptomatic grapevine by PCR also was improved when xylem samples were tested (66.7%) compared with whole-tissue samples (23.1%). There were no differences in frequency of detection of X. fastidiosa in symptomatic grapevines by ELISA or PCR dependent upon sample collection method. Assays of xylem fluid samples did not improve detection of X. fastidiosa in symptomatic or asymptomatic oleander compared with assays...Continue Reading

References

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Citations

Feb 25, 2005·BioTechniques·Blake Bextine, Thomas A Miller
Jan 29, 2013·Applied and Environmental Microbiology·Leonard NunneyRichard Stouthamer
Mar 1, 2006·Plant Disease·Rayda K KrellCarmen Gispert
Feb 22, 2005·Current Microbiology·Blake BextineThomas A Miller
May 1, 2020·Applications in Plant Sciences·Bendangchuchang LongcharMuthappa Senthil-Kumar
May 15, 2019·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (PLH)Stephen Parnell

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