Comparison on in vitro fertilized bovine embryos cultured in KSOM or SOF and cryopreserved by slow freezing or vitrification

Theriogenology
Tshimangadzo Lucky NedambaleX Yang

Abstract

The objectives of this study were to identify an improved in vitro cell-free embryo culture system and to compare post-warming development of in vitro produced (IVP) bovine embryos following vitrification versus slow freezing. In Experiment 1, non-selected presumptive zygotes were randomly allocated to four medium treatments without co-culture: (1) SOF + 5% FCS for 9 days; (2) KSOM + 0.1% BSA for 4 days and then KSOM + 1% BSA to Day 9; (3) SOF + 5% FCS for 4 days and then KSOM + 1% BSA to Day 9; and (4) KSOM + 0.1% BSA for 4 days and then SOF + 5% FCS to Day 9. Treatment 4 (sequential KSOM-SOF culture system) improved (P > 0.05) morulae (47%), early blastocysts (26%), Day-7 blastocysts (36%), cell numbers, as well as total hatching rate (79%) compared to KSOM alone (Treatment 2). Embryos cultured in KSOM + BSA alone developed slowly and most of them hatched late on Day 9, compared to other treatments. In Experiment 2, the sequential KSOM-SOF culture system was used and Day-7 blastocysts were subjected to following cryopreservation comparison: (1) vitrification (VS3a, 6.5 M glycerol); or (2) slow freezing (1.36 M glycerol). Warmed embryos were cultured in SOF with 7.5% FCS. Higher embryo development and hatching rates (P < 0.05)...Continue Reading

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Citations

Jan 15, 2013·Journal of Assisted Reproduction and Genetics·Shu HashimotoYoshiharu Morimoto
Apr 7, 2011·The Journal of Reproduction and Development·Yasushi InabaKei Imai
Jan 29, 2008·Zygote : the Biology of Gametes and Early Embryos·Juan Carlos Flores-AlonsoNéstor M Delgado
Jul 16, 2004·Biology of Reproduction·Tshimangadzo L NedambaleX Cindy Tian
Mar 17, 2011·Zygote : the Biology of Gametes and Early Embryos·Alessandra Corallo NicacioJose Antonio Visintin
Dec 1, 2011·Cryobiology·Rouhollah FathiFatemeh Hassani
Feb 4, 2006·Reproductive Biomedicine Online·Mark G LarmanDavid K Gardner
Jun 23, 2006·Reproductive Biomedicine Online·Bogdan OrasanuCatherine Racowsky
Dec 19, 2015·Animal Reproduction Science·Alisha GuptaMuhammad Anzar
Feb 27, 2007·Animal Reproduction Science·Kwang Taek LimByeong Chun Lee
Apr 20, 2006·Animal Reproduction Science·R M Garcia-GarciaP Lonergan
Mar 18, 2015·Theriogenology·Roberta C GasparFlavia L Lopes
Jan 27, 2015·Journal of Assisted Reproduction and Genetics·Hideyuki IwahataYoshiharu Morimoto
Jan 5, 2011·Fertility and Sterility·Mark G Larman, David K Gardner
May 24, 2014·Biology of Reproduction·Priscila Ramos-IbeasMiguel Ángel Ramírez
Nov 26, 2019·Animal : an International Journal of Animal Bioscience·L B FerréP J Ross
Nov 16, 2016·Annals of Biomedical Engineering·Marcia A M M FerrazBart M Gadella
Jan 28, 2021·Veterinary Medicine and Science·Carina BlaschkaJens Tetens
Nov 7, 2020·Molecular Reproduction and Development·Thamiris V MarsicoMateus J Sudano
Feb 20, 2021·Reproduction, Fertility, and Development·Emilio Gutierrez-CastilloZongliang Jiang

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