PMID: 377235Jan 1, 1979Paper

Comparison on the structure and transcriptional capability of growing phase and stationary yeast chromatin: a model for reversible gene activation

Nucleic Acids Research
D Lohr, G Ide

Abstract

We have compared the structure of intra-nuclear and isolated chromatin from logarithmically growing yeast cells to chromatin from cells which had entered the stationary phase and ceased growing. Both chromatins show a similar nucleosomal repeat pattern, 160 bp repeat size, with staphylococcal nuclease and similar variability in repeat sizes within the genome. DNase I produces the same ladder (less than 120 b) and a quite similar extended ladder (120-300 b) which shows that both chromatins have phased nucleosomes. However, the rate of DNase I digestion of growing phase is greater than in stationary. Functionally speaking, growing phase nuclei are 5-20 times as active in the rate of endogenous transcription (all three polymerases are involved). The transcriptional and DNase I susceptibility differences noted in nuclei are maintained in sucrose gradient isolated oligonucleosomes and mononucleosomes from the two states.

References

Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·A Prunell, R D Kornberg
Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·R D Camerini-OteroG Felsenfeld
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K Tatchell, K E Van Holde
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D LohrK E Van Holde
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D A NelsonR L Rill
Jan 1, 1977·Annual Review of Biochemistry·R D Kornberg
Nov 1, 1977·Cell·D LohrK E Van Holde
Jan 12, 1978·Nature·G Felsenfeld
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·P ValenzuelaW J Rutter
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L D Schultz, B D Hall
Sep 1, 1977·Nucleic Acids Research·J M Gottesfeld, P J Butler
Sep 3, 1976·Science·H Weintraub, M Groudine
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E Lacy, R Axel
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A Garel, R Axel
Jul 1, 1975·Biochemistry·B Sollner-Webb, G Felsenfeld
Sep 1, 1974·Analytical Biochemistry·R McMaster-Kaye, J S Kaye
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R AdmanB D Hall

❮ Previous
Next ❯

Citations

Nov 1, 1981·Current Genetics·G J Ide, C A Saunders
Jun 1, 1989·Molecular & General Genetics : MGG·J F Martínez-GarcíaJ E Pérez-Ortín
Jun 1, 1981·Molecular and Cellular Endocrinology·K S KatulaS Sridhara
Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S Smart-NixonT E Webb
Sep 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Georgia SchäferHugh-G Patterton
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D Lohr, K E Van Holde
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D Lohr, L Hereford
Mar 1, 1982·The Journal of Cell Biology·M NagyG Hervé
Jul 30, 2004·Nucleic Acids Research·Antonio Rodríguez-CamposMiguel Beato
Mar 3, 1986·FEBS Letters·F EstruchL Franco
Dec 15, 1980·Journal of Molecular Biology·W HörzH G Zachau
Jul 24, 2015·Nucleic Acids Research·Mark T RutledgeJames R Broach
Jun 25, 2016·FEBS Letters·Andrew LeungChristopher J Nelson
May 6, 2019·Current Genetics·Sarah G Swygert, Toshio Tsukiyama
Jun 24, 1983·Biochimica Et Biophysica Acta·D A Prentice, L R Gurley
Dec 22, 1983·Biochimica Et Biophysica Acta·D A PrenticeL R Gurley
Mar 1, 1996·Molecular and Cellular Biology·M M SmithP C Megee
Feb 1, 1992·Genes & Development·J H WrightV A Zakian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.