Comparisons of genetic variability and genome structure among mosquito strains selected for refractoriness to a malaria parasite

The Journal of Heredity
G YanD W Severson

Abstract

Restriction fragment length polymorphism (RFLP) markers were used to evaluate Aedes aegypti genome structure and genetic variability within and between substrains selected for different levels of refractoriness to the malaria parasite, Plasmodium gallinaceum. The MOYO-R substrain was previously selected for complete refractoriness and the MOYO-IS substrain for intermediate susceptibility from the Moyo-In-Dry (MOYO) strain by selective inbreeding (F = 0.5). Eighteen mapped RFLP markers were used to provide coverage of the mosquito genome. The two substrains showed reduced genetic diversity compared with the MOYO strain, including significant reductions in mean heterozygosity, number of alleles per locus, and proportion of polymorphic loci. Genetic differentiation between the two substrains was statistically significant, as reflected by differences in allele frequencies. Significant pairwise linkage disequillbrium among the RFLP loci was detected in all three strains, most evidently in the MOYO strain. This is surprising because the RFLP loci examined are separated by large map distances, and therefore linkage disequilibrium should decay to zero after many generations of laboratory culture. Our hypothesis to explain this phenomen...Continue Reading

Citations

Nov 16, 2013·Die Naturwissenschaften·Christopher D PullMark J F Brown
Feb 13, 2001·Insect Biochemistry and Molecular Biology·C Lowenberger
Nov 30, 2012·Proceedings. Biological Sciences·Louis LambrechtsChristine Chevillon
Oct 18, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Hinrich SchulenburgMichael T Siva-Jothy
Jan 5, 2001·Genetics·K L Ayres, D J Balding

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