Comparisons of several biological and physicochemical properties of human leukocyte interferons produced my human leukocytes and by E. coli

Gene
W E StewartC Weissmann

Abstract

Human interferon (IFN) prepared from virus-induced human leukocyte suspensions (leukocyte-derived interferon) was compared to the IFN extracted from Escherichia coli harboring a human interferon-alpha cDNA hybrid plasmid (Hif-SN35-AH-L6). E coli-derived IFN was 20 to 50 times more active than leukocyte-derived IFN on heterologous bovine, feline, murine and guinea pig cells, relative to the activity on human cells. After partial purification by affinity chromatography on an anti-human lymphoblastoid IFN antibody column, the IFN was analyzed by SDS-polyacrylamide gel electrophoresis. While leukocyte-derived IFN gave a heterogeneous pattern with major peaks of activity of 24000 and 19000 daltons, E. coli-derived IFN gave a heterogeneous peak of activity at about 17-18000 daltons. The leading edge of leukocyte-derived IFN in SDS-polyacrylamide gels was significantly more active on bovine cells than on human cells and coincided in mobility with E. coli-derived IFN, which was also much more active on bone than on human cells. After reduction with mercaptoethanol in SDS, the E. coli-derived IFN lost no activity, whereas the leukocyte-derived IFN lost about 90% of its activity. After reduction, E. coli-derived IFN migrated in SDS-polya...Continue Reading

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W E StewartK Cantell
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K C ZoonC B Anfinsen
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T M RobertsM Ptashne
Jan 1, 1977·Scandinavian Journal of Immunology·K Berg
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J D SipeE de Maeyer
Jan 1, 1974·Preparative Biochemistry·W E StewartE De Clercq
Jan 1, 1974·The Journal of General Virology·K E Mogensen, K Cantell
Jul 10, 1980·Nature

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Citations

Jan 1, 1987·Cancer Metastasis Reviews·M Shearer, J Taylor-Papadimitriou
Jan 1, 1982·Archives of Virology·F H Sarkar, S L Gupta
Jun 1, 1995·Pharmacology & Therapeutics·M A Horisberger, S Di Marco
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M StreuliC Weissmann
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E De MaeyerK Atherton
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·M D Edge, R Camble
Feb 1, 1981·Journal of Interferon Research·I A BraudeW E Stewart
Jan 1, 1985·Journal of Interferon Research·N B RajP M Pitha
Aug 1, 1982·Archives of Biochemistry and Biophysics·F H SarkarL A Pickering
Aug 20, 1981·Nature·M D EdgeA F Markham
Jan 28, 2010·Pharmaceuticals·Manfred W BeilharzJoseph M Cummins
Jan 1, 1982·Journal of Interferon Research·D A Eppstein
Sep 30, 1982·Biochimica Et Biophysica Acta·M Rubinstein
Nov 13, 1981·Science·P B SehgalI A Braude
Sep 19, 1980·Science·M G MasucciC Weissmann

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