Comparisons of sperm quality, morphometry and function among human sperm populations recovered via SpermPrep II filtration, swim-up and Percoll density gradient methods

Andrologia
Y YamamotoN Sofikitis

Abstract

The purpose of this study was to compare the morphology/morphometry and fertilizing capacity of human spermatozoa recovered via swim-up method, Percoll density gradient method, and SpermPrep II filtration method. Thirty-three ejaculates were equally divided into 2 aliquots. Aliquot 1 was processed via the direct swim-up method, whereas aliquot 2 was filtered via a SpermPrep II column. The Percoll density gradient method was compared with the SpermPrep II method in a similar protocol using 43 ejaculates. Sperm populations recovered via the SpermPrep II filtration method showed significantly higher hypoosmotic swelling test results, acrosin profiles, and percentage of hyperactivated spermatozoa than sperm fractions recovered by the swim-up method. Furthermore, significant differences were found in most of sperm morphometric parameters between the above sperm populations. However, sperm fractions recovered via the SpermPrep II method did not show significantly different values for these same tests and for most of sperm morphometric parameters compared to the Percoll density gradient method. These results suggest that the SpermPrep II filtration and Percoll density gradient method are equally efficient in isolating sperm subpopulat...Continue Reading

References

Apr 1, 1992·The Tohoku Journal of Experimental Medicine·N SofikitisN Terakawa
Oct 1, 1989·Fertility and Sterility·K P KatayamaR S Jeyendran
Jul 1, 1987·Journal of Andrology·J C GoodpastureL J Zaneveld
Oct 1, 1970·Journal of Reproduction and Fertility·R Yanagimachi
Mar 1, 1982·The Journal of Experimental Zoology·A D Fleming, R Yanagimachi
Dec 1, 1994·International Journal of Andrology·J F GriveauD Le Lannou

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Citations

Jan 28, 2020·Reproduction in Domestic Animals = Zuchthygiene·Katarzyna AndraszekMarta Kuchta-Gładysz
Jun 12, 2009·International Journal of Andrology·Fotios DimitriadisNikolaos Sofikitis

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