Comparisons of the responses of anterior and posterior human adult male bladder neck smooth muscle to in vitro stimulation

BJU International
John F BoltonGary N A Sibley

Abstract

To evaluate differing methods of stimulation on strips of human bladder neck smooth muscle and compare muscle taken from the anterior and posterior aspects. Samples of adult human male bladder neck muscle were obtained from patients undergoing open radical prostatectomy. Muscle was taken from either the anterior or posterior (nine and six patients, respectively) aspects of the bladder neck. Muscle strips dissected from these samples were suspended in the Brading-Sibley organ bath. The strips were superfused with 100 mm KCl-enriched Krebs' solution for 4 min to determine viability. This allowed experimentation on 17 strips from the anterior aspect of the bladder neck and 13 from the posterior bladder neck. These remaining strips were then superfused either with various concentrations (x10(-7) to x10(-3)m) of carbachol or noradrenaline in Krebs' solution, for 15 s. A further set of strips (eight from anterior, six from posterior) was suspended and responses to electrical field stimulation (EFS) with varying parameters were measured. Each EFS experiment was repeated after a 15 min exposure to 10(-3)m atropine, and again after a 15 min exposure 10(-7)m tetrodotoxin (TTX). Tension responses produced in these series of experiments we...Continue Reading

References

Oct 1, 1978·British Journal of Urology·J D Jenkins, N H Allen
Apr 1, 1985·British Journal of Urology·H Hedlund, A Ek
Apr 1, 1994·British Journal of Urology·J M Gil-VernetJ M Gil-Vernet
May 8, 2000·Neurourology and Urodynamics·A GroutzD C Chaikin

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