Compartmental stress responses correlate with cell survival in bystander effects induced by the DNA damage agent, bleomycin

Mutation Research
Diana SavuNicoleta Moisoi

Abstract

Physical or chemical stress applied to a cell system trigger a signal cascade that is transmitted to the neighboring cell population in a process known as bystander effect. Despite its wide occurrence in biological systems this phenomenon is mainly documented in cancer treatments. Thus understanding whether the bystander effect acts as an adaptive priming element for the neighboring cells or a sensitization factor is critical in designing treatment strategies. Here we characterize the bystander effects induced by bleomycin, a DNA-damaging agent, and compartmental stress responses associated with this phenomenon. Mouse fibroblasts were treated with increasing concentrations of bleomycin and assessed for DNA damage, cell death and induction of compartmental stress response (endoplasmic reticulum, mitochondrial and cytoplasmic stress). Preconditioned media were used to analyze bystander damage using the same end-points. Bleomycin induced bystander response was reflected primarily in increased DNA damage. This was dependent on the concentration of bleomycin and time of media conditioning. Interestingly, we found that ROS but not NO are involved in the transmission of the bystander effect. Consistent transcriptional down-regulation ...Continue Reading

References

Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·T K HeiC Waldren
Aug 29, 2002·The EMBO Journal·Quan ZhaoNicholas J Hoogenraad
Jul 31, 2003·Drugs·Uzair B Chaudhary, Jason R Haldas
Aug 30, 2003·Oncogene·Paul DentSteven Grant
Nov 9, 2004·Mutation Research·Philip J CoatesEric G Wright
Mar 10, 2005·International Journal of Cancer. Journal International Du Cancer·Chunlin ShaoKevin M Prise
Dec 2, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lutz ThonDieter Adam
Mar 25, 2006·International Journal of Cancer. Journal International Du Cancer·Aicha DemidemJean Claude Madelmont
Jul 7, 2006·Annual Review of Cell and Developmental Biology·Sebastián BernalesPeter Walter
Mar 1, 2007·Journal of Radiation Research·Nobuyuki HamadaYasuhiko Kobayashi
Jun 5, 2007·Nature Protocols·Michael Fenech
Jun 26, 2007·Journal of Radiation Research·Genro KashinoMasami Watanabe
Sep 7, 2007·Physics in Medicine and Biology·E Claridge MackonisM Jackson
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jul 23, 2008·The Journal of Pharmacy and Pharmacology·Tom K HeiCharles R Geard
Nov 4, 2008·International Journal of Radiation Biology·Tadayuki HaradaMasami Watanabe
Apr 21, 2009·Nature Reviews. Cancer·Kevin M Prise, Joe M O'Sullivan
Jun 3, 2009·Mutation Research·Rajalakshmi S AsurJames D Tucker
Oct 20, 2009·European Journal of Pharmacology·Joanna Rzeszowska-WolnyMaria Widel
Dec 26, 2009·Mutation Research·Rajalakshmi AsurJames D Tucker
Oct 23, 2010·Molecular Cell·Keith A SpriggsAnne E Willis
Oct 23, 2010·Molecular Cell·Alexander BuchbergerThomas Sommer
Oct 23, 2010·Molecular Cell·Klaus RichterJohannes Buchner
Nov 5, 2010·Journal of Cell Science·Cole M Haynes, David Ron
Dec 15, 2010·Current Molecular Pharmacology·Nobuyuki HamadaMasanori Tomita
Apr 27, 2012·Physiological Reviews·John M Kyriakis, Joseph Avruch
May 25, 2012·Nature·Julie A MorenoGiovanna R Mallucci

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Citations

Mar 31, 2016·Radiation and Environmental Biophysics·Mihaela TemelieDiana Savu
Nov 22, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Priya SamuelDavid Raul Francisco Carter
Aug 18, 2018·Oxidative Medicine and Cellular Longevity·Mihaela TemelieNicoleta Moisoi
Mar 20, 2021·DNA Repair·Teresa BrooksAndrew J Massey

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