Compartmentalization of allogeneic T-cell responses in the bone marrow and spleen of humanized NOD/SCID mice containing activated human resident myeloid dendritic cells

Experimental Hematology
Slavica VuckovicDerek N J Hart

Abstract

Human allogeneic (allo)-T-cell responses within recipient lymphoid tissues and the degree to which they are altered in the presence of activated tissue-resident dendritic cells (DC) remain unknown. This study examined allo-T-cell recruitment and the early allo-T-cell responses that occur in the bone marrow (BM) and spleen (SP) of humanized (hu) nonobese diabetic (NOD)/severe combined immunodeficient (SCID) recipients containing activated human tissue-resident myeloid DC (MDC). Human naïve allo-T cells were transferred into polyinosinic:polycytidylic acid [poly(I:C)]-treated or untreated huNOD/SCID recipients containing human tissue-resident DC derived from transplanted CD34(+) cells. Activation of human tissue-resident MDC mediated by poly(I:C) treatment, recruitment, proliferation, and effector differentiation of allo-T cells in the BM and SP of huNOD/SCID recipients were analyzed in vivo by flow cytometry. Poly(I:C) treatment induced transient activation of human MDC within a maximum of 8 hours, as evidenced in the BM by an increased proportion of MDC-expressing CD86 while in the SP by MDC expressing CD86 and producing interleukin-12. Poly(I:C)-pretreated huNOD/SCID recipients showed changes in the recruitment of allo-T cells...Continue Reading

Citations

Jan 22, 2013·Human Pathology·Sherif A RezkLawrence M Weiss
Apr 18, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·David J Schneider, Heidi S Taatjes-Sommer
Sep 13, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lisa M FreemanSlavica Vuckovic
Jan 24, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yitian DingKristen J Radford
Oct 28, 2019·The Journal of Infectious Diseases·Jordana G A Coelho-Dos-ReisMoriya Tsuji
Mar 20, 2019·Frontiers in Immunology·Xi Zen YapMeredith O'Keeffe

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