PMID: 1204636Dec 1, 1975Paper

Compartmentation of acetyl-coA in rat-liver mitochondria

European Journal of Biochemistry
G von Glutz, P Walter

Abstract

The ratio of the specific radioactivities of 3-hydroxybutyrate: citrate was determined in rat liver mitochondria which were incubated in the presence of [1-14C]palmitate, pyruvate, bicarbonate, ATP, phosphate and malonate. Without compartmentation this ratio would maximally be 2, however, under our conditions values of 2.5-3.7 were observed. In further experiments with mitochondria, the sensitivity of pyruvate carboxylase for acetyl-CoA produced from various precursors was tested. It was found that acetyl-CoA produced from L-acetylcarnitine or by oxidation from either pyruvate, octanoate or palmitylcarnitine but not from leucine led to a stimulation of pyruvate carboxylation. These results demonstrate a compartmentation of acetyl-CoA in liver mitochondria. The further finding that different mitochondrial fractions showed varying ratios of specific radioactivities of 3-hydroxybutyrate:citrate indicates that the observed compartmentation may be explained by the existence of different types of mitochondria with varying enzyme patterns and acetyl-CoA pools.

References

Oct 17, 1972·European Journal of Biochemistry·J W Stucki, P Walter
Apr 1, 1974·European Journal of Biochemistry·G WeissW Seubert
Aug 1, 1972·The Biochemical Journal·M A Wilson, J Cascarano
May 1, 1972·European Journal of Biochemistry·J W StuckiP Walter

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Citations

Feb 1, 1978·European Journal of Biochemistry·M Lopes-CardozoS G van den Bergh
Oct 5, 1990·European Journal of Biochemistry·S PassarellaE Quagliariello
Aug 1, 1986·Biological Chemistry Hoppe-Seyler·D Häussinger
Jan 1, 1989·The Journal of Pharmacology and Experimental Therapeutics·M R LakshmanP J Coutlakis
Aug 1, 1989·Clinical Physiology·N J Fuller, M Elia

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