Compatibility of recombinant tissue plasminogen activator (rtPA) and aflibercept or ranibizumab coapplied for neovascular age-related macular degeneration with submacular haemorrhage

The British Journal of Ophthalmology
Alexa KlettnerJ Hillenkamp

Abstract

Subretinal coapplication of recombinant tissue plasminogen activator (rtPA) and vascular endothelial growth factor (VEGF)-antagonists is a new treatment option for age-related macular degeneration complicated by submacular haemorrhage. Here, we investigate the compatibility of rtPA and aflibercept or ranibizumab in vitro because intraoperatively, rtPA or rtPA-induced plasmin may cleave aflibercept or ranibizumab. Aflibercept and ranibizumab, respectively, were incubated with rtPA or plasmin, separated in gel electrophoresis and stained with Coomassie or silver. The antiangiogenic activity of the VEGF-antagonists was quantified by VEGF-ELISA after incubation with the supernatant of primary porcine retinal pigment epithelium cell cultures. In electrophoresis, ranibizumab displayed no additional fragments when it was coapplied with rtPA or plasmin. Its VEGF-inhibiting efficacy remained unchanged in coapplication with rtPA with or without blood, or plasmin. rtPA did not cleave or functionally compromise aflibercept. When aflibercept was coapplied with plasmin, electrophoresis displayed additional bands in Coomassie (30 kDa, 27 kDa, 19 kDa, 15 kDa) and silver staining (31 kDa, 26 kDa, 21 kDa, 19 kDa, 15 kDa). While at a clinical dos...Continue Reading

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Citations

Feb 18, 2016·Movement Disorders : Official Journal of the Movement Disorder Society·Alexa KlettnerSusanne A Schneider
Apr 21, 2016·Progress in Retinal and Eye Research·Chee Wai WongChui Ming Gemmy Cheung
Nov 18, 2017·European Journal of Ophthalmology·Handan BardakSemrin Timlioglu
Nov 20, 2016·Movement Disorders : Official Journal of the Movement Disorder Society·Alexa KlettnerSusanne A Schneider
Aug 9, 2020·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·Lars-Olof HattenbachArgyrios Chronopoulos
Dec 26, 2017·Current Ophthalmology Reports·Joon-Bom KimAjay E Kuriyan

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