Compatible Solute Synthesis and Import by the Moderate Halophile Spiribacter salinus : Physiology and Genomics

Frontiers in Microbiology
María J LeónErhard Bremer

Abstract

Members of the genus Spiribacter are found worldwide and are abundant in ecosystems possessing intermediate salinities between seawater and saturated salt concentrations. Spiribacter salinus M19-40 is the type species of this genus and its first cultivated representative. In the habitats of S. salinus M19-40, high salinity is a key determinant for growth and we therefore focused on the cellular adjustment strategy to this persistent environmental challenge. We coupled these experimental studies to the in silico mining of the genome sequence of this moderate halophile with respect to systems allowing this bacterium to control its potassium and sodium pools, and its ability to import and synthesize compatible solutes. S. salinus M19-40 produces enhanced levels of the compatible solute ectoine, both under optimal and growth-challenging salt concentrations, but the genes encoding the corresponding biosynthetic enzymes are not organized in a canonical ectABC operon. Instead, they are scrambled (ectAC; ectB) and are physically separated from each other on the S. salinus M19-40 genome. Genomes of many phylogenetically related bacteria also exhibit a non-canonical organization of the ect genes. S. salinus M19-40 also synthesizes trehal...Continue Reading

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Citations

Sep 30, 2020·Critical Reviews in Microbiology·Satish KumarYogesh Shouche
Jan 11, 2020·Frontiers in Microbiology·Alexandra A RichterErhard Bremer
Oct 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sun-Wook Jeong, Yong Jun Choi
Nov 6, 2020·Journal of Microbiology and Biotechnology·Ye-Lim ParkYung-Hun Yang
Nov 7, 2020·Gene·Dylan Casey, Roy D Sleator
Apr 14, 2021·Environmental Microbiology·Thomas P ThompsonBrendan F Gilmore

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Datasets Mentioned

BETA
CP005963
CBV43892.1
OBX36375

Methods Mentioned

BETA
Assay
environmental stresses
gene knock-out

Software Mentioned

Swiss
ImageQuant TM
dnadist
Model
PyMOL
OpenLAB
BLAST
IMG
PHYLIP

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