Compatible Solutes in the Thermophilic Bacteria Rhodothermus marinus and "Thermus thermophilus".

Applied and Environmental Microbiology
O C NunesHelena Santos

Abstract

(sup13)C nuclear magnetic resonance spectroscopy and (sup1)H nuclear magnetic resonance spectroscopy were used to identify and quantify the organic solutes of several strains of halophilic or halotolerant thermophilic bacteria. Two strains of Rhodothermus marinus and four strains of "Thermus thermophilus" grown in complex medium containing NaCl were examined. 2-O-Mannosylglycerate was a major compatible solute in all strains: the Thermus strains accumulated the (beta)-anomer only, whereas both anomers were found in R. marinus. 2-O-(beta)-mannosylglycerate and 2-O-(alpha)-mannosylglycerate were the major compatible solutes in R. marinus. The former was the predominant solute in cells grown in 2.0 and 4.0% NaCl-containing medium, while the latter was the predominant compatible solute at higher salinities. Glutamate, trehalose, and glucose were also present as minor components. The intracellular K(sup+) concentration, as determined by (sup39)K nuclear magnetic resonance spectroscopy, in R. marinus increased with salinity and was sufficient to balance the negative charges of the mannosylglycerate. In addition to 2-O-(beta)-mannosylglycerate, trehalose was a major compatible solute of "T. thermophilus." 2-O-(beta)-Mannosylglycerate ...Continue Reading

References

Dec 1, 1976·Bacteriological Reviews·A D Brown
Jan 1, 1991·Annual Review of Microbiology·L N Csonka, A D Hanson
Jul 1, 1990·Journal of Bacteriology·D McLagganW Epstein
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K R SowersM F Roberts
Feb 1, 1990·Applied and Environmental Microbiology·D E RobertsonD R Boone
Apr 1, 1986·Journal of Bacteriology·S GrotheJ M Wood
Jun 1, 1994·Journal of Bacteriology·R CiullaM F Roberts
Feb 1, 1990·Applied and Environmental Microbiology·J L Botsford, T A Lewis
Aug 1, 1992·Applied and Environmental Microbiology·D E RobertsonM F Roberts
Oct 1, 1994·Applied and Environmental Microbiology·R A CiullaM F Roberts

❮ Previous
Next ❯

Citations

Apr 9, 1998·Bioscience, Biotechnology, and Biochemistry·X XuS Yamazaki
Sep 11, 2002·Environmental Microbiology·Helena Santos, Milton S da Costa
Nov 23, 2006·Molecular Microbiology·Teca Calcagno GalvãoDavid Cánovas
Oct 27, 2011·Environmental Microbiology·Luís G GonçalvesHelena Santos
Aug 16, 2005·FEBS Letters·Mathumai KanapathipillaiChan Beum Park
Sep 6, 2011·International Journal of Systematic and Evolutionary Microbiology·Luciana AlbuquerqueMilton S da Costa
Aug 3, 2005·Extremophiles : Life Under Extreme Conditions·Snaedis H BjornsdottirJakob K Kristjansson
Apr 20, 2004·FEBS Letters·Anubhav AroraChan Beum Park
Apr 2, 2005·Biotechnology and Bioengineering·Chanki Ha, Chan Beum Park
Jan 22, 2009·Extremophiles : Life Under Extreme Conditions·Felipe CavaJosé Berenguer
Aug 30, 2007·Extremophiles : Life Under Extreme Conditions·Susana AlaricoMilton S da Costa
Jan 18, 2017·Archives of Microbiology·Preeti Ranawat, Seema Rawat
Aug 12, 2014·Extremophiles : Life Under Extreme Conditions·Nuno BorgesHelena Santos
Jun 24, 2015·Extremophiles : Life Under Extreme Conditions·Mohamed JebbarPhilippe Oger
Nov 26, 2010·International Journal of Systematic and Evolutionary Microbiology·Luciana AlbuquerqueMilton S da Costa
Feb 21, 2018·International Journal of Systematic and Evolutionary Microbiology·Luciana AlbuquerqueMilton S da Costa
Feb 6, 2018·Advanced Pharmaceutical Bulletin·Niloofar BazazzadeganHamid Reza Khorram Khorshid
Sep 30, 2018·The Journal of Microbiology·Pedro A GalleguillosD Barrie Johnson
Mar 1, 1997·Applied and Environmental Microbiology·L O MartinsH Santos
Mar 15, 2006·Applied and Environmental Microbiology·A RamosH Santos
Feb 26, 2014·Journal of Proteome Research·Joseph R PittmanJanet R Donaldson
Nov 28, 2007·Biochemical and Biophysical Research Communications·Mathumai KanapathipillaiChan Beum Park
Aug 7, 2021·Chemistry : a European Journal·Pedro FerreiraMaria J Ramos
Jul 5, 2008·Systematic and Applied Microbiology·Nuno Empadinhas, Milton S da Costa

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.