PMID: 8613989Apr 26, 1996Paper

Compensating effects of opposing changes in putrescine (2+) and K+ concentrations on lac repressor-lac operator binding: in vitro thermodynamic analysis and in vivo relevance

Journal of Molecular Biology
Michael W CappM T Record

Abstract

Ion concentrations (K+, Glu-) in the cytoplasm of growing Escherichia coli cells increase strongly with increases in the osmolarity of a defined growth medium. While in vitro experiments demonstrate that the extent of protein-nucleic acid interactions (PNAI) depends critically on salt concentration, in vivo measurements indicate that cells maintain a relatively constant extent of PNAI independent of the osmolarity of growth. How do cells buffer PNAI against changes in the cytoplasmic environment? At high osmolarity, the increase in macromolecular crowding which accompanies the reduction in amount of cytoplasmic water in growing cells appears quantitatively sufficient to compensate for the increase in [K+]. At low osmolarity, however, changes in crowding appear to be insufficient to compensate for changes in [K+], and additional mechanisms must be involved. Here we report quantitative determinations of in vivo total concentrations of polyamines (putrescine(2+), spermidine(3+)) as a function of osmolarity (OsM) of growth, and in vitro binding data on the effects of putrescine concentration on a specific PNAI (lac repressor-lac operator) as a function of [K+]. The total concentration of putrescine in cytoplasmic water decreases at...Continue Reading

Citations

Oct 16, 1999·The Journal of Biological Chemistry·C M Falcon, K S Matthews
Jan 25, 2017·Nucleic Acids Research·Robert J Trachman, David E Draper
Dec 24, 2004·Journal of Bioinformatics and Computational Biology·X-M ZhuP Ao
Jun 15, 2011·Annual Review of Microbiology·Janet M Wood
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Andrew WhiteheadFernando Galvez
May 29, 2012·Journal of Bacteriology·Barbara L Schneider, Larry Reitzer
Aug 25, 2004·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Ke WangGuang-Hua Gao
Mar 4, 2021·Molecular Ecology·Molly A AlbeckerMichael W McCoy
Oct 24, 2009·Biochemistry·Steven D BrownM G Finn
May 19, 1998·Trends in Biochemical Sciences·M T RecordH J Guttman
Jul 20, 2002·Current Opinion in Structural Biology·Ruth M Saecker, M Thomas Record
Aug 1, 2009·EcoSal Plus·Karlheinz AltendorfJanet M Wood
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·J M Wood
Jun 24, 2004·Journal of Molecular Biology·Steven A Mauro, Gerald B Koudelka

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